Presence of high-level DNA copy number gains in gastric carcinoma and severely dysplastic adenomas but not in moderately dysplastic adenomas
Presence of high-level DNA copy number gains in gastric carcinoma and severely dysplastic adenomas but not in moderately dysplastic adenomas
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DOI：
10.1016/s0165-4608(98)00092-2
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发表时间：
1998-11-01
影响因子：
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通讯作者：
Knuutila, S
中科院分区：
文献类型：
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作者：
Kokkola, A;Monni, O;Knuutila, S
我们的目的是调查胃腺瘤中 DNA 拷贝数变化的存在，并确定可能在胃癌发生中发挥作用的变化。采用比较基因组杂交技术研究了 16 例胃腺瘤患者和 22 例肠型胃癌患者肿瘤中 DNA 拷贝数的变化。 44% 的腺瘤病例和 86% 的肠型胃癌病例中发现 DNA 拷贝数变化。平均而言，增加比减少更常见（腺瘤中为 0.9 vs. 0.5，癌中为 4.1 vs. 1.8）。在腺瘤中，最常见的增益涉及3例8号染色体，2例检测到7号和20q染色体增益。最常见的损失发生在 5q（3 次）。只有严重不典型增生的腺瘤才在 13 号染色体、17cen-q22 和 20q12-ter 处检测到高水平扩增。在胃癌中，最常见的增益出现在 20q (55%)、17q12-q21 (41%) 和 8q (41%)，最常见的缺失出现在 18q (43%) 和 4q (32%)。在 20q（3 个肿瘤）、17cen-q21（3 个肿瘤）、2p（1 个肿瘤）和 18q（1 个肿瘤）处观察到高水平扩增。这些发现表明，不典型增生的进展与较高水平的 DIVA 拷贝数增加（例如，17q 和 20q 的增加）相关，这通常在肠型胃癌中观察到。此外，结果支持腺瘤先于癌症的假设。 (C) 爱思唯尔科学公司，1998 年。
Our aim was to investigate the presence of DNA copy number changes in gastric adenomas and to identify the changes that may play a role in gastric carcinogenesis. DNA copy number changes in 16 patients with gastric adenoma and in 22 tumors from patients with intestinal type gastric carcinomas were studied by using comparative,genomic hybridization. DNA copy number changes were found in 44% of the adenoma cases and in 86% of the intestinal type gastric carcinomas. On average, gains were more common than losses (0.9 vs. 0.5 in adenomas and 4.1 vs. 1.8 in carcinomas). In adenomas, the most common gains involved chromosome 8 in 3 cases, and gain of chromosome 7 and 20q was detected in 2 cases. The most frequent losses were observed at 5q (three times). Only adenomas with severe dysplasia showed high-level amplifications that were detected at chromosome 13, 17cen-q22, and 20q12-ter. In gastric cancer, the most common gains were detected at 20q (55%), 17q12-q21 (41%), and 8q (41%), and the most common losses were detected at 18q (43%) and 4q (32%). High-level amplifications were observed at 20q (3 tumors), 17cen-q21 (3 tumors), 2p (1 tumor), and 18q (1 tumor). These findings suggest that the progression of dysplasia is associated with higher levels of DIVA copy number increase (e.g., the gains at 17q and 20q), which were typically observed in the intestinal type gastric cancer. Furthermore, the results support the hypothesis that adenoma precedes cancer. (C) Elsevier Science Inc., 1998.