Two pathways of transferrin recycling evident in a variant of mouse LMTK- cells.
Two pathways of transferrin recycling evident in a variant of mouse LMTK- cells.
复制标题
小鼠 LMTK 细胞变体中明显有两种转铁蛋白循环途径。
DOI：
10.1007/bf01534713
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Draper,RK
中科院分区：
文献类型：
--
作者：
O'Keefe,DO;Draper,RK
We describe here the properties of a variant cell line, termed AF192, selected by exposing mouse LMTK}-cells to a cytotoxic form of transferrin prepared by conjugating transferrin to diphtheria toxin. AF192 cells were mildly resistant to the transferrindiphtheria toxin conjugate and were cross-resistant to the protein toxins modeccin, abrin, ricin, and Pseudomonas aeruginosa exotoxin A. AF192 cells had an aberrant transferrin cycle characterized by an ∼50% reduction in the rate of iron uptake from diferric transferrin, an ∼25% reduction in the number of surface transferrin receptors, and a time course for transferrin recycling that resolved into two apparent first-order rate processes. The aberrant transferrin cycle was not the result of a failure of endocytosed transferrin to discharge iron; rather, part, but not all, of the transferrin taken up by AF192 cells was diverted to an intracellular site from which it was recycled very slowly.
