Escherichia coli induces DNA double-strand breaks in eukaryotic cells
Escherichia coli induces DNA double-strand breaks in eukaryotic cells
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DOI：
10.1126/science.1127059
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发表时间：
2006-08-11
期刊：
影响因子：
56.9
通讯作者：
Oswald, Eric
中科院分区：
文献类型：
--
作者：
Nougayrede, Jean-Philippe;Homburg, Stefan;Oswald, Eric
系统发育组B2的肠内和肠外致病性大肠杆菌瞬时感染真核细胞阻断有丝分裂并诱导巨红细胞增多。这种特性与编码巨大模块化非核糖体肽和聚酮酶的广泛分布的基因组岛有关。接触E。表达该基因簇的大肠杆菌引起DNA双链断裂和DNA损伤检查点途径的激活，导致细胞周期停滞并最终导致细胞死亡。在大肠杆菌中发现杂合肽-聚酮基因毒素。大肠杆菌将改变我们对致病机理和致病性的看法，并开辟新的生物技术应用。
Transient infection of eukaryotic cells with commensal and extraintestinal pathogenic Escherichia coli of phylogenetic group B2 blocks mitosis and induces megalocytosis. This trait is linked to a widely spread genomic island that encodes giant modular nonribosomal peptide and polyketide synthases. Contact with E. coli expressing this gene cluster causes DNA double-strand breaks and activation of the DNA damage checkpoint pathway, leading to cell cycle arrest and eventually to cell death. Discovery of hybrid peptide-polyketide genotoxins in E. coli will change our view on pathogenesis and commensalism and open new biotechnological applications.