Safety of intra-articular use of atelocollagen for enhanced tissue repair.
Safety of intra-articular use of atelocollagen for enhanced tissue repair.
复制标题
DOI：
10.2174/1874325001206010231
复制
发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Murray MM
中科院分区：
文献类型：
--
作者：
Magarian EM;Vavken P;Connolly SA;Mastrangelo AN;Murray MM
胶原蛋白是关节内组织工程中的重要生物材料，但其安全性仍存在问题。我们假设，在猪模型中，添加I型胶原用于前交叉韧带（ACL）的一期修复可能会在15周后导致局部和全身反应，表现为关节积液、滑膜增厚、关节内和全身白细胞计数升高。此外，添加血小板浓缩物可能会加重该反应。横切18只猪髂动脉，并使用缝线（n=6）、胶原海绵（n=6）或胶原-血小板复合物（CPC; n=6）进行修复。12个完整的对侧膝关节作为对照（n=12）。在胶原蛋白治疗的膝关节中没有观察到显著的滑膜增厚或关节积液。手术治疗膝关节和完整膝关节之间的滑液白细胞计数无显著差异，外周血白细胞计数也无差异。在膝关节中添加血小板浓缩物导致血清IL-1β水平降低，但血清TNF-α水平在组间无显著差异。总之，胶原蛋白的存在，有或没有添加血小板，没有增加手术后的局部或全身炎症反应，表明I型胶原蛋白在膝关节中使用是安全的。
Collagen is an important biomaterial in intra-articular tissue engineering, but there are unanswered questions about its safety. We hypothesize that the addition of type-I-collagen for primary repair of the Anterior Cruciate Ligament (ACL) might result in a local and systemic reaction in a porcine model after 15 weeks as demonstrated by joint effusion, synovial thickening, elevated intraarticular and systemic leukocyte counts. Further, this reaction might be aggravated by the addition of a platelet concentrate. Eighteen porcine ACLs were transected and repaired with either sutures (n=6), a collagen sponge (n=6), or a collagen-platelet-composite (CPC; n=6). Twelve intact contralateral knees served as controls (n=12). No significant synovial thickening or joint effusion was seen in the collagen-treated knees. Synovial fluid leukocyte counts showed no significant differences between surgically treated and intact knees, and no differences were seen in leukocyte counts of the peripheral blood. The addition of a platelet concentrate to the knee joint resulted in lower serum levels of IL-1β, but serum levels of TNF-α were not significantly different between groups. In conclusion, the presence of collagen, with or without added platelets, did not increase the local or systemic inflammatory reactions following surgery, suggesting that Type I collagen is safe to use in the knee joint.