Rapid, noninvasive screening for perturbations of metabolism and plant growth using chlorophyll fluorescence imaging
Rapid, noninvasive screening for perturbations of metabolism and plant growth using chlorophyll fluorescence imaging
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DOI：
10.1104/pp.102.018093
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发表时间：
2003-06-01
期刊：
影响因子：
7.4
通讯作者：
Baker, NR
中科院分区：
文献类型：
--
作者：
Barbagallo, RP;Oxborough, K;Baker, NR
描述了一种快速、非侵入性的技术，包括叶绿素荧光参数成像，用于检测幼苗叶片代谢和生长的扰动。拟南芥幼苗生长在96孔微量滴定板4天，然后用8种除草剂与不同的作用模式，以诱导扰动的范围内不同的代谢过程。来自生长在微量滴定板上的96个幼苗的叶绿素荧光发射的成像使得能够快速且同时地产生每个孔中的植物的许多荧光参数的图像。除草剂诱导的扰动代谢，甚至在代谢反应不直接与光合代谢，检测荧光参数的图像中的变化相当之前，观察到任何视觉效果对幼苗生长。通过测量在96孔板中生长的植物的图像中叶绿素荧光发射的面积来评估幼苗生长。幼苗生长下降直接关系到除草剂诱导的成像叶绿素荧光参数的变化。这种快速筛选技术在单子叶植物物种的代谢扰动的适用性证明了通过处理Agrostis tenuis幼苗与咪唑烟酸，支链氨基酸合成的抑制剂。
A rapid, noninvasive technique involving imaging of chlorophyll fluorescence parameters for detecting perturbations of leaf metabolism and growth in seedlings is described. Arabidopsis seedlings were grown in 96-well microtitre plates for 4 d and then treated with eight herbicides with differing modes of action to induce perturbations in a range of different metabolic processes. Imaging of chlorophyll fluorescence emissions from 96 seedlings growing on a microtitre plate enabled images of a number of fluorescence parameters to be rapidly and simultaneously produced for the plants in each well. Herbicide-induced perturbations in metabolism, even in metabolic reactions not directly associated with photosynthetic metabolism, were detected from the changes in the images of fluorescence parameters considerably before any visual effects on seedling growth were observed. Evaluations of seedling growth were made from measurements of the area of chlorophyll fluorescence emission in images of plants growing in the 96-well plates. Decreased seedling growth related directly to herbicide-induced changes in the imaged chlorophyll fluorescence parameters. The applicability of this rapid-screening technique for metabolic perturbations in monocotyledonous species was demonstrated by treating Agrostis tenuis seedlings with Imazapyr, an inhibitor of branched-chain amino acid synthesis.