The induction of H3K9 methylation by PIWIL4 at the p16Ink4a locus
The induction of H3K9 methylation by PIWIL4 at the p16Ink4a locus
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DOI：
10.1016/j.bbrc.2007.05.136
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发表时间：
2007-08-03
影响因子：
3.1
通讯作者：
Takai, Daiya
中科院分区：
文献类型：
--
作者：
Sugimoto, Keiki;Kage, Hidenori;Takai, Daiya
随着小rna介导的表观遗传修饰的发现，表观遗传学领域取得了新的进展。然而，人们对其中的关键蛋白质知之甚少。在这里，我们报告了人类piwi样家族是一个候选蛋白，参与负责染色质重塑的途径。PIWI-like家族蛋白以flag融合蛋白的形式表达，形成一个庞大的体，定位于核外周。PIWI-like 4 （PIWIL4）是PIWI-like家族中唯一在人体组织中普遍表达的成员，瞬时转染PIWI-like 4可诱导p16(Ink4a) （CDKN2A）位点的H3赖氨酸9甲基化。组蛋白甲基化水平升高导致p16（Ik4a）基因下调。这些结果提示PIWIL4在人体细胞染色质修饰通路中发挥重要作用。(c) 2007爱思唯尔公司版权所有。
The field of epigenetics has made progress by the identification of the small RNA-mediated epigenetic modification. However, little is known about the key proteins. Here, we report that the human PIWI-like family is a candidate protein that is involved in the pathway responsible for chromatin remodeling. The PIWI-like family proteins, expressed as the Flag-fusion proteins, formed a bulky body and localized to the nuclear periphery. Transient transfection of PIWI-like 4 (PIWIL4), only member of the PIWI-like family that was ubiquitously expressed in human tissues, induced historic H3 lysine 9 methylation at the p16(Ink4a) (CDKN2A) locus. The elevated level of histone methylation resulted in the downregulation of the p16(Ik4a) gene. These results suggest PIWIL4 plays important roles in the chromatin-modifying pathway in human somatic cells. (c) 2007 Elsevier Inc. All rights reserved.