Metabolomics evaluation of serum markers for cachexia and their intra-day variation in patients with advanced pancreatic cancer.
Metabolomics evaluation of serum markers for cachexia and their intra-day variation in patients with advanced pancreatic cancer.
复制标题
DOI：
10.1371/journal.pone.0113259
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Minami H
中科院分区：
文献类型：
--
作者：
Fujiwara Y;Kobayashi T;Chayahara N;Imamura Y;Toyoda M;Kiyota N;Mukohara T;Nishiumi S;Azuma T;Yoshida M;Minami H
癌症恶病质是一种以进行性体重减轻和肌肉萎缩为特征的多因素综合征。利用代谢组学，我们研究了伴有恶病质的晚期胰腺癌患者的血清标志物及其日内变化。患者分为两组：有恶病质组和无恶病质组。分别于上午6:30、11:30、下午4:30和晚上9:30采集血样进行代谢组学分析，测定两组血清IL-6、TNF-α和瘦素水平并进行比较。然后评估日内变化。共纳入21例患者。在恶病质组（n = 9）， 6个月的中位体重减轻率高于非恶病质组（n = 12），表现较差，厌食症更严重。每一种代谢物水平在白天都有很大的变化，其中一些在两组之间表现出显著差异。在所有测量点上，恶病质组的副黄嘌呤水平仍然明显较低。恶病质组中位IL-6、TNF-α水平升高，瘦素浓度降低，但差异无统计学意义。一些代谢产物和一些血清学标志物水平受到癌症恶病质的影响。虽然恶病质患者的副黄嘌呤水平一直较低，但我们发现许多代谢物显示出较大的日内和日间变化，因此在代谢组学研究中可能有必要关注日内变化。
Cancer cachexia is a multifactorial syndrome characterized by progressive loss of weight and muscle atrophy. Using metabolomics, we investigated serum markers and their intra-day variation in advanced pancreatic cancer patients with cachexia. Patients were enrolled in two groups: those with or without cachexia. Blood samples collected at 6:30 AM, 11:30 AM, 4:30 PM, and 9:30 PM were analyzed using metabolomics, and serum levels of IL-6, TNF-α, and leptin were measured and compared between the two groups. Intra-day variation was then evaluated. Twenty-one patients were enrolled in total. In the cachexia group (n = 9), median body weight loss rate over 6 months was greater, performance status was poorer, and anorexia was more severe than in the non-cachexia group (n = 12). Each metabolites level showed substantial intra-day variation, and some of them displayed significant differences between the two groups. Levels of paraxanthine remained markedly lower in the cohort with cachexia at all measurement points. Besides, median IL-6 and TNF-α levels appeared higher and leptin concentration appeared lower in the cachexia group, albeit without statistical significance. Some metabolites and some serological marker levels were affected by cancer cachexia. Although paraxanthine levels were consistently lower in patients with cachexia, we identified that many metabolites indicated large intra- and inter-day variation and that it might be necessary to pay attention to intra-day variation in metabolomics research.
登录
查看更多内容
影响因子：
3.6
作者：
Nishiumi, Shin;Shinohara, Masakazu;Yoshida, Masaru
通讯作者：
Yoshida, Masaru
影响因子：
4.7
作者：
Maccio, Antonio;Madeddu, Clelia;Mantovani, Giovanni
通讯作者：
Mantovani, Giovanni
影响因子：
3.3
作者：
HETZLER, RK;KNOWLTON, RG;PERKINS, RM
通讯作者：
PERKINS, RM
影响因子：
3.7
作者：
Nishiumi S;Kobayashi T;Ikeda A;Yoshie T;Kibi M;Izumi Y;Okuno T;Hayashi N;Kawano S;Takenawa T;Azuma T;Yoshida M
通讯作者：
Yoshida M
影响因子：
3.2
作者：
Bachmann, Jeannine;Heiligensetzer, Mathias;Martignoni, Marc E.
通讯作者：
Martignoni, Marc E.