The apicoplast of Haemoproteus columbae: A comparative study of this organelle genome in Haemosporida
The apicoplast of Haemoproteus columbae: A comparative study of this organelle genome in Haemosporida
复制标题
DOI：
10.1016/j.ympev.2021.107185
复制
发表时间：
2021-05-08
影响因子：
4.1
通讯作者：
Matta, Nubia E.
中科院分区：
文献类型：
--
作者：
Cepeda, Axl S.;Pacheco, M. Andreina;Matta, Nubia E.
顶复虫是寄生原生动物的一个门；其中包括血孢子目，一种媒介传播的寄生虫，包括引起疟疾的寄生虫（疟原虫属）。大多数顶复合体物种具有非光合质体或顶质体。鉴于其独特的代谢途径，这种细胞器被认为是疟疾治疗的靶标。尽管它很重要，但缺乏完整的顶质体基因组数据阻碍了比较研究。本研究利用新一代测序技术获得了嗜血杆菌（Haemoproteus）柱状体顶质体基因组（谱系HAECOL1）。该基因组被包括在与其他质体的比较分析中。这个29.8 kb的环状基因组与疟原虫的结构相同。它富含A + T(87.7%)，与疟原虫的A + T含量相当，但处于较高的水平（85.5-87.2%）。正如预期的那样，考虑到其较高的A + T含量，同义密码子使用率（RSCU）和有效密码子数量（ENc）表现出适度的密码子偏置。一些顶质体基因具有系统发育信号。然而，与线粒体基因不同，单基因系统发育在最近分化的血孢子虫分支中得到的支持较少。柱孢顶质体基因组表明，顶质体功能可能在整个血孢属中是保守的。这种寄生虫可以作为研究非哺乳动物系统中这种细胞器的模型。
Apicomplexa is a phylum of parasitic protozoa; among them are the order Haemosporida, vector-borne parasites that include those that cause malaria (genus Plasmodium). Most Apicomplexa species have a non-photosynthetic plastid or apicoplast. Given its unique metabolic pathways, this organelle is considered a target for malaria therapeutics. Regardless of its importance, there is a paucity of complete apicoplast genome data hindering comparative studies. Here, the Haemoproteus (Haemoproteus) columbae apicoplast genome (lineage HAECOL1) was obtained using next-generation sequencing. This genome was included in a comparative analysis with other plastids. This 29.8 kb circular genome shares the same structure found in Plasmodium parasites. It is A + T rich (87.7%), comparable but at the higher end of A + T content observed in Plasmodium species (85.5-87.2%). As expected, considering its high A + T content, the synonymous codon usage (RSCU) and the effective number of codons (ENc) showed a moderate codon bias. Several apicoplast genes have a phylogenetic signal. However, unlike mitochondrial genes, single-gene phylogenies have low support in haemosporidian clades that diverged recently. The H. columbae apicoplast genome suggests that the apicoplast function may be conserved across Haemosporida. This parasite could be a model to study this organelle in a non-mammalian system.