Detection of Fusarium spp. in plants with monoclonal antibody
Detection of Fusarium spp. in plants with monoclonal antibody
复制标题
DOI：
10.3186/jjphytopath.61.311
复制
发表时间：
1995-01-01
期刊：
影响因子：
--
通讯作者：
Yamaguchi, Isamu
中科院分区：
文献类型：
--
作者：
Arie, Tsutomu;Hayashi, Yoshio;Yamaguchi, Isamu
利用抗镰刀菌单克隆抗体AP 19 -2对镰刀菌进行血清学检测。植物组织中的尖孢。F.用单克隆抗体AP 19 -2和FITC标记的抗鼠IgM-羊IgG联合免疫荧光试验（IFA），对番茄和日本益母草的茎、冠、根中的尖孢镰刀菌进行了有效的检测，表明IFA是一种有效的观察镰刀菌行为的方法。在植物组织中。作为该单抗的另一种免疫学检测，将直接组织印迹免疫结合试验（DT-IBA）应用于番茄和黄瓜植株的茎或冠的横切片。在本试验中，感染F.尖孢菌在4 h内呈阳性反应，接种后14 ~ 21 d内，病原菌在无菌植株的导管中出现。因此，DT-IBA是一种快速、准确诊断镰刀菌的实用方法。
A monoclonal antibody (MoAb) AP19-2, which reacts specifically to members of the genus Fusarium, was tested for serological detection of F. oxysporum in plant tissue. Mycelia of F. oxysporum in infected stems, crowns, and roots of tomato and Japanese honewort plants were effectively detected by immunofluorescence assay (IFA) with the combination of MoAb AP19-2 and FITC-conjugated anti-mouse IgM-sheep IgG indicating that IFA is a useful toot for the observation of behavior of Fusarium spp. in plant tissue. As another immunological assay with this MoAb, direct tissue-blotted immunobinding assay (DT-IBA) was applied to transverse sections from steins or crowns of tomato and cucumber plants. In this assay, samples of plants infected with F. oxysporum showed positive reaction within 4 hr. In addition, the pathogen residing in vessels of infected, but symptomless plants was observed within 14 to 21 days after inoculation. Thus, DT-IBA is thought to be a practical method for quick and accurate diagnosis of Fusarium spp.