Iron, nitric oxide, and myeloperoxidase in asthmatic patients
Iron, nitric oxide, and myeloperoxidase in asthmatic patients
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DOI：
10.1023/b:biry.0000026205.89894.25
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发表时间：
2004-04-01
影响因子：
2.8
通讯作者：
Demir, T
中科院分区：
文献类型：
--
作者：
Ekmekci, OB;Donma, O;Demir, T
测定支气管哮喘患者血浆一氧化氮（NO）、髓过氧化物酶（MPO）和铁（Fe）水平。解释了哮喘不同阶段这些参数之间的关系。评价了它们与支气管哮喘患者气道炎症的关系以及在病理过程中的作用和贡献。共有62名个体，32名哮喘患者和30名对照者被纳入本研究范围。血浆一氧化氮代谢产物（NOx）和MPO和铁的水平分别通过Griess反应，ELISA和自动TPTZ（2，4，6-三[2-吡啶基]-5-三嗪）方法测定。在哮喘患者中，血浆NOx、MPO和Fe浓度分别为133 +/- 13 μ M、95 +/- 20 ng/ml和159 +/- 20 mug/dl;在对照组中，这些值分别为82 +/- 11 μ M、62 +/- 11 ng/ml和96 9 mug/dl。哮喘患者血浆MPO（p > 0.05）、NOx（p < 0.01）和Fe（p < 0.01）浓度升高。考虑到事实上，NO调节MPO的催化活性，并诱导血红素加氧酶的表达作为重要的贡献者的机制，导致游离铁的释放，它的结论是，在哮喘组中观察到的NOx，MPO，铁水平升高的行为在一个协调的方式，似乎参与了哮喘的发病机制。
Plasma nitric oxide (NO), mycloperoxidase (MPO), and iron (Fe) levels were determined in bronchial asthma. The relations among these parameters in different stages of asthma were interpreted. Their association with airway inflammation observed in patients with bronchial asthma as well as the roles and the: contributions to the pathological processes were evaluated. A total of 62 individuals, 32 asthmatics and 30 controls, were included into the scope of this study. Plasma nitric oxide metabolites (NOx) and MPO and Fe levels were determined by the Griess reaction, ELISA, and the automated TPTZ (2,4,6-tri[2-pyridyl]-5-triazine) method, respectively. In the asthmatic individuals, plasma NOx, MPO, and Fe concentrations were 133 +/- 13 muM, 95 +/- 20 ng/ml, and 159 +/- 20 mug/dl, respectively; in the control group these values were 82 +/- 11 muM, 62 +/- 11 ng/ml, and 96 9 mug/dl. Increased values were detected for plasma MPO (p > 0.05), NOx (p < 0.01), and Fe (p < 0.01) concentrations in asthmatic individuals. Considering the facts that NO modulates the catalytic activity of MPO and induces the expression of heme oxygenase as important contributors to the mechanisms causing free Fe release, it is concluded that elevated NOx, MPO, and Fe levels observed in the asthmatic group act in a concerted manner and appear to be involved in the pathogenesis of asthma.