RNA-seq analysis provides insight into reprogramming of culm development in Zizania latifolia induced by Ustilago esculenta
RNA-seq analysis provides insight into reprogramming of culm development in Zizania latifolia induced by Ustilago esculenta
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DOI：
10.1007/s11103-017-0658-9
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
De-Ping Guo 郭得平
中科院分区：
文献类型：
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作者：
Zhi-Dan Wang 王志丹;Ning Yan 闫宁;Zheng-Hong Wang 王征鸿;Xiao-Huan Zhang 张晓焕;Jing-Ze Zhang 张敬泽;Hui-Min Xue 薛惠民;De-Ping Guo 郭得平
The smut fungus Ustilago esculenta can induce culm gall in Zizania latifolia, which is used as a vegetable in Asian countries. However, the underlying olecular.mechanism of culm gall formation is still unclear. To characterize the processes underlying this host-fungus association, we performed transcriptomic and expression profiling analyses of culms from Z. latifolia infected by the fungus U. esculenta. Transcriptomic analysis detected U. esculenta induced differential expression of 19,033 and 17,669 genes in Jiaobai (JB) and Huijiao (HJ) type of gall, respectively. Additionally, to detect the potential gall inducing genes,expression profiles of infected culms collected at −7, 1 and 10 DAS of culm gall development were analyzed. Compared to control, we detected 8089 genes (4389 up-regulated, 3700 down-regulated) and 5251 genes (3121 up-regulated, 2130 down-regulated) were differentially expressed in JB and HJ, respectively. And we identified 376 host and 187 fungal candidate genes that showed stage-specific expression pattern, which are possibly responsible for gall formation at the initial and later phases, respectively. Our results indicated that cytokinins play more prominent roles in regulating gall formation than do auxins. Together, our work provides general implications for the understanding of gene regulatory networks for culm gall development in Z. latifolia, and.potential targets for genetic manipulation to improve the future yield of this crop.
