Effects of alcohol on the symptoms of gouty arthritis and taxonomic structure of gut microbiota in C57BL/6 mice.
Effects of alcohol on the symptoms of gouty arthritis and taxonomic structure of gut microbiota in C57BL/6 mice.
复制标题
酒精对C57BL/6小鼠痛风性关节炎症状及肠道微生物群分类结构的影响。
DOI：
10.3389/fmicb.2023.1257701
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发表时间：
2023
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
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作者：
痛风是由血清尿酸(UA)水平升高引起的一种急性关节炎，其患病率一直在迅速上升。酗酒可能会导致一系列健康问题。多项证据表明，酒精摄入影响痛风的发生和发展，而肠道微生物区系在痛风的发生发展中起着重要作用，长期饮酒可能会影响肠道微生物区系的稳定性。本研究旨在基于肠道微生物区系，探讨不同浓度酒精摄入对痛风性关节炎的影响。采用C57BL/6小鼠注射尿酸单钠(MSU)建立的急性痛风性关节炎模型，观察不同浓度酒精对痛风性关节炎的影响。结果表明，高酒精摄入不仅加重痛风小鼠关节肿胀和疼痛，升高尿酸、肿瘤坏死因子α、白介素1β、白介素6水平，而且对痛风小鼠肠道微生物区系的组成和结构也有显著影响。两种关键微生物Parasutterella和Alistipe可通过脂多糖的生物合成、核黄素代谢、苯丙氨酸代谢以及精氨酸和脯氨酸代谢来加重痛风症状。综上所述，我们的研究表明，高浓度酒精改变了MSU晶体诱导的痛风小鼠的肠道微生物区系结构，这可能通过增强促炎途径来加剧痛风症状。
Gout is an acute arthritis caused by the elevated levels of serum uric acid (UA), and its prevalence has been rapidly increasing. Alcohol abuse could lead to a series of health problems. Multiple pieces of evidence suggest that alcohol intake affects the development and progression of gout, while the gut microbiota plays an important role in the development of gout and the long-term alcohol consumption could affect the stability of the gut microbiota. This study aimed to explore the effects of alcohol intake at different concentrations on gouty arthritis based on the gut microbiota. We investigated the effects of different concentrations of alcohol on gouty arthritis in mouse models of acute gouty arthritis established by injection of monosodium urate (MSU) crystals into C57BL/6 mice. The results indicated that the high-alcohol consumption not only exacerbated joint swelling and pain, increased the levels of UA, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6), but also showed dramatic effects on the composition and structure of the gut microbiota in gouty mice. Two key microorganisms, Parasutterella and Alistipes, could aggravate gout symptoms through lipopolysaccharide biosynthesis, riboflavin metabolism, phenylalanine metabolism, and arginine and proline metabolisms. In conclusion, our study suggested that high-concentrations of alcohol altered the gut microbiota structure in gouty mice induced by MSU crystals, which could exacerbate gouty symptoms by enhancing pro-inflammatory pathways.