Immunocytochemical and immunohistochemical application of monoclonal antibodies against peroxisomal biogenesis factor 14.
Immunocytochemical and immunohistochemical application of monoclonal antibodies against peroxisomal biogenesis factor 14.
复制标题
抗过氧化物酶体生物发生因子 14 的单克隆抗体的免疫细胞化学和免疫组织化学应用。
作者：
Shaojuan Yu;Fuli Wang;Kui Zhang;Yun Zhang;Kun Yang;Man;Chao;Boquan Jin
PEX14是一种完整的膜蛋白，是蛋白质对接到过氧化物酶体上所必需的，是一种双功能蛋白质，既可以作为转录共抑制因子，也可以作为多肽运输调节剂。进一步的研究表明，PEX14是唯一一种在过氧化体形成和选择性降解中具有独特双重功能的过氧化物素。它的RNA转录本普遍表达；然而，由于缺乏适合免疫组织化学染色的单抗，因此没有关于PEX14在蛋白质水平上的表达谱的数据，从而阻碍了对其功能的研究。在本研究中，我们制备了一种可用于免疫细胞化学和免疫组织化学的单抗，并研究了PEX14在正常和恶性人类组织中的表达。与其RNA转录本的普遍表达相反，PEX14蛋白在正常人体组织中不表达，包括肝、脾、肺、直肠、脑、前列腺、乳腺、卵巢和胃。在某些恶性肿瘤中，PEX14蛋白表达显著上调。本文提供了PEX14在蛋白质水平的表达谱的第一批数据，这将进一步加深我们对其功能的理解。
PEX14 is an integral membrane protein essential for protein docking onto the peroxisomes and is a bi-functional protein capable of acting as a transcriptional co-repressor and a polypeptide transport modulator. Further studies showed that PEX14 is the sole peroxin that has a unique dual function in peroxisome formation and selective degradation. Its RNA transcripts are ubiquitously expressed; there is, however, no data on the expression profiles of PEX14 at the protein level due to a lack of MAbs suitable for immunohistochemical staining, thus hindering studies on its functions. In the present study, we generated one MAb that could be used in immunocytochemistry and immunohistochemistry and investigated PEX14 expression in normal and malignant human tissues. In contrast to the ubiquitous expression of its RNA transcripts, there is no PEX14 protein expression in normal human tissue, including liver, spleen, lung, rectum, brain, prostate, breast, ovary, and stomach. Protein expression of PEX14 was dramatically upregulated in some malignancies. Presented here are the first data on the expression profiles of PEX14 at the protein level, which will further our understanding of its functions.