Interaction of Fusobacterium nucleatum 191 with human peripheral blood lymphocytes.
Interaction of Fusobacterium nucleatum 191 with human peripheral blood lymphocytes.
复制标题
具核梭杆菌 191 与人外周血淋巴细胞的相互作用。
DOI：
10.1111/j.1600-0765.1990.tb00929.x
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发表时间：
1990
影响因子：
3.5
通讯作者：
Mangan,DF
中科院分区：
文献类型：
--
作者：
Tuttle,RS;Mangan,DF
具核梭杆菌经常从牙周病患者的龈下菌斑中大量分离。这些细菌对几种宿主组织的粘附是凝集素样的，因为它被半乳糖、乳糖和N-乙酰基-半乳糖胺抑制。自F.本研究的目的是探讨F.如果是，则确定凝集素样相互作用是否促进淋巴细胞活化。F.通过凝集试验、扫描电子显微镜和使用荧光素或[3H]标记的细菌证实了nucleatumstrain 191对淋巴细胞的作用。在每淋巴细胞约35个细菌时达到粘附饱和。结果表明，与富含T细胞的亚群相比，富含B细胞的亚群具有更高的粘附率。N-乙酰基-半乳糖胺、乳糖>半乳糖、α-和β-甲基半乳糖苷抑制粘附，但100 mM葡萄糖、甘露糖、N-乙酰基-葡萄糖胺或其他测试的糖不抑制粘附。当与细菌预孵育并洗涤以去除弱粘附和非粘附细菌时，淋巴细胞经历有丝分裂。低浓度F. N-乙酰基-半乳糖胺可减少核仁，但不能完全消除。然而，N-乙酰半乳糖胺对高浓度细菌预孵育诱导的有丝分裂无影响。这些结果表明，F. nucleatum促进淋巴细胞的活化，特别是在低浓度的细菌。
Fusobacterium nucleatumzis frequently isolated in high numbers from the subgingival plaque of patients with periodontal diseases. Adherence of these bacteria to several host tissues is lectin‐like in that it is inhibited by galactose, lactose, and N‐acetyl‐galactosamine. SinceF. nucleatumhas the capacity to activate human peripheral blood lymphocytes, the purpose of this study was to determine whetherF. nucleatumadheres to lymphocytes and, if so, to determine whether lectin‐like interactions facilitate lymphocyte activation. Adherence ofF. nucleatumstrain 191 to lymphocytes was confirmed by agglutination assays, scanning electron microscopy, and by using fluorescein‐ or [3H]‐labeled bacteria. Saturation of adherence was reached at about 35 bacteria per lymphocyte.F. nucleatumadhered to > 90% of the lymphocytes, and adhered in higher numbers to B cell‐enriched subpopulations than to T cell‐enriched subpopulations. Adherence was inhibited by N‐acetyl‐galactosamine, lactose > galactose, alpha‐ and beta‐methyl galactoside, but not by 100 mM glucose, mannose, N‐acetyl‐glucosamine, or other sugars tested. Lymphocytes underwent mitogenesis when preincubated with bacteria and washed to remove weakly adherent and non‐adherent bacteria. Mitogenesis induced by low concentrations ofF. nucleatumwas reduced, but not completely eliminated, by N‐acetyl‐galactosamine. However, N‐acetyl‐galactosamine had no effect on mitogenesis induced by preincubation with high concentrations of bacteria. These results suggest that adherence ofF. nucleatumfacilitates activation of lymphocytes, particularly at low concentrations of bacteria.