Stable and direct coating of fibronectin-derived Leu-Asp-Val peptide on ePTFE using one-pot tyrosine oxidation for endothelial cell adhesion
Stable and direct coating of fibronectin-derived Leu-Asp-Val peptide on ePTFE using one-pot tyrosine oxidation for endothelial cell adhesion
复制标题
使用一锅酪氨酸氧化将纤连蛋白衍生的 Leu-Asp-Val 肽稳定直接涂覆在 ePTFE 上以促进内皮细胞粘附
DOI：
10.1016/j.colsurfb.2022.112576
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Yamaoka T
中科院分区：
文献类型：
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作者：
Kakinoki S;Nishioka S;Yuki A;Yamaoka T
膨胀聚四氟乙烯（ePTFE）由于其柔韧性、生物稳定性和不粘连性被广泛用于临床应用，例如制造血液接触植入装置。多肽修饰是进一步改善ePTFE功能的有效策略。然而，聚四氟乙烯的化学稳定性使其难以用肽修饰。在这项研究中，我们报道了一种简单的方法，通过锚定序列Tyr-Lys-Tyr-Lys-Tyr-Lys （YK3）在ePTFE表面致密而稳定地包裹生物功能肽。以YK3为锚点，结合α4β1整合素的配体序列Leu-Asp-Val (LDV)，成功地通过一锅氧化将肽（YK3-LDV）包被ePTFE接枝上。通过YK3-LDV涂层在ePTFE上构建的肽层稳定，耐高浓度盐和表面活性剂水溶液的广泛洗涤。YK3-LDV涂层促进内皮细胞对ePTFE的体外粘附。此外，在植入ePTFE贴片的大鼠颈动脉模型中，YK3-LDV涂层加速了新生内膜样组织的体内形成。
Expanded polytetrafluoroethylene (ePTFE) is widely used in clinical applications, such as in the manufacture of blood-contacting implantable devices, owing to its flexibility, biostability, and non-adhesiveness. Modification with peptides is an effective strategy to further improve the ePTFE function. However, the chemical stability of PTFE makes it difficult to modify with peptides. In this study, we reported a simple method for the dense and stable coating of biofunctional peptides on the ePTFE surface through the anchor sequence, Tyr-Lys-Tyr-Lys-Tyr-Lys (YK3). A peptide (YK3-LDV) incorporating the YK3 anchor and a ligand sequence for α4β1integrin, Leu-Asp-Val (LDV), was successfully coated on ePTFE grafts through one-pot oxidation. The peptide layer constructed via YK3-LDV coating on ePTFE was stable and resistant to extensive washing by aqueous solutions of highly concentrated salts and surfactants. YK3-LDV coating promoted the in vitro adhesion of endothelial cells to ePTFE. Furthermore, YK3-LDV coating accelerated the in vivo formation of neointima-like tissue in a rat model with an ePTFE patch implanted into the carotid artery.