ULTRASTRUCTURAL ALTERATION OF CYTOLYTIC LYMPHOCYTES-T FOLLOWING THEIR INTERACTION WITH TARGET-CELLS .1. HYPERTROPHY AND CHANGE OF ORIENTATION OF GOLGI APPARATUS
ULTRASTRUCTURAL ALTERATION OF CYTOLYTIC LYMPHOCYTES-T FOLLOWING THEIR INTERACTION WITH TARGET-CELLS .1. HYPERTROPHY AND CHANGE OF ORIENTATION OF GOLGI APPARATUS
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DOI：
10.1016/0008-8749(78)90324-6
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发表时间：
1978-01-01
影响因子：
4.3
通讯作者：
BYKOVSKY, AF
中科院分区：
文献类型：
--
作者：
BYKOVSKAJA, SN;RYTENKO, AN;BYKOVSKY, AF
在相互作用开始后的不同时期，研究了粘附于靶细胞[小鼠白血病L1210细胞]表面的溶细胞性T [胸腺衍生]淋巴细胞的超微结构。孵育15 min后，高尔基体、池和液泡数量增加。孵育30分钟后，高尔基体变得朝向接触区域。如果有几个淋巴细胞粘附在一个靶细胞上，则每个淋巴细胞的高尔基体都朝向接触区。如果1个淋巴细胞同时粘附于2个靶细胞，则其高尔基体朝向两个靶细胞。30-60 min后，高尔基体内形成巨大空泡，并移向淋巴细胞质膜，空泡内的内含物移向溶细胞性T淋巴细胞与靶细胞之间的细胞间隙。高尔基体的肥大和方向改变所需的时间可能代表了一个中型和小型杀伤淋巴细胞的动员步骤。
The ultrastructure of cytolytic T [thymus derived] lymphocytes adhered to the surface of target cells [mouse leukemia L1210 cells] was investigated at different periods after start of interaction. A 15 min incubation led to increase of number of Golgi apparatus cisternae and vacuoles. After 30 min incubation Golgi apparatus became oriented to the contact area. If several lymphocytes adhered to 1 target cell the Golgi apparatus of each of them was oriented toward the contact area. If 1 lymphocyte adhered simultaneously to 2 target cells its Golgi apparatus was oriented toward both target cells. Giant Golgi apparatus vacuoles were formed 30-60 min later, then moved to the plasma membrane of the lymphocyte and then the content of those vacuoles moved to the intercellular space between a cytolytic T lymphocyte and a target cell. The period required for the hypertrophy and change of orientation of Golgi apparatus may represent the mobilization step of a medium-sized and small killer lymphocyte.