DETECTION OF MULTIPLE BLOOD FEEDING IN AEDES-AEGYPTI (DIPTERA, CULICIDAE) DURING A SINGLE GONOTROPHIC CYCLE USING A HISTOLOGIC TECHNIQUE
DETECTION OF MULTIPLE BLOOD FEEDING IN AEDES-AEGYPTI (DIPTERA, CULICIDAE) DURING A SINGLE GONOTROPHIC CYCLE USING A HISTOLOGIC TECHNIQUE
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DOI：
10.1093/jmedent/30.1.94
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发表时间：
1993-01-01
影响因子：
2.1
通讯作者：
EDMAN, JD
中科院分区：
文献类型：
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作者：
SCOTT, TW;CLARK, GG;EDMAN, JD
我们评估了一种组织学技术，其在检测埃及伊蚊（L.）在一个单一的生殖营养循环中。为了使程序标准化，我们进行了一项实验室研究，其中166只蚊子在已知的时间间隔内吸入两次血餐。当两餐间隔为1 ~ ≤ 24 h，第二餐至注视时间为0 ~ 12 h时，80%（78/98）的多餐被检出，在此范围外，仅34%（23/68）的多餐被检出。总的来说，61%（101/166）的双餐被检测到。对96例充血型Ae.通过抽吸从波多黎各的圣胡安的房屋内收集的埃及人表明，50%的埃及人吸收了多餐。大多数野外捕获的蚊子在捕获前一天吃了最后一顿饭，大多数多食者连续几天喂了两次。消化的血液，或血红素，第一顿饭和两餐之间的物理分离的黑线是最有用的组织学参数检测多喂养野生Ae。埃及人。多次喂养与卵母细胞发育的高级阶段的关联表明，在收集的时候，大多数Ae。来自研究地点的埃及人在每个生殖营养周期中进食两次。我们的结论是，虽然这是劳动密集型，组织学检查是一个适当的技术，纵向，社区范围内的调查，多喂养的AE。埃及人。
We evaluated a histologic technique for its usefulness in detecting multiple blood feeding by Aedes aegypti (L.) in a single gonotrophic cycle. To standardize the procedure, we carried out a laboratory study in which 166 mosquitoes imbibed two blood meals at known intervals. Eighty percent (78/98) of the multiple meals were detected when the interval between meals was from 1 to less-than-or-equal-to 24 h and the time from the second meal to fixation ranged from 0 to 12 hr. At intervals outside this range, only 34% (23/68) of the multiple meals were detected. Overall, 61% (101/166) of the double meals were detected. Examination of 96 engorged Ae. aegypti collected by aspiration from inside houses in San Juan, Puerto Rico, indicated that 50% had imbibed multiple meals. Most wild-caught mosquitoes took their last meal the day before capture, and most multiple feeders fed twice on consecutive days. A dark line of digested blood, or heme, around the first meal and a physical separation between meals were the most useful histologic parameters for detecting multiple feeding in wild Ae. aegypti. An association of multiple feeding with advanced stages of oocyte development suggests that, at the time of collection, most Ae. aegypti from the study site had fed twice in each gonotrophic cycle. We conclude that, although it is labor intensive, histologic examination is an appropriate technique for a longitudinal, community-wide survey of multiple feeding by Ae. aegypti.