Interleukin-9 receptor expression in asthmatic airways in vivo
Interleukin-9 receptor expression in asthmatic airways in vivo
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DOI：
10.1007/s004080000018
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发表时间：
2000-05-01
期刊：
影响因子：
5
通讯作者：
Erzurum, SC
中科院分区：
文献类型：
--
作者：
Bhathena, PR;Comhair, SAA;Erzurum, SC
气道壁的炎症是哮喘的一个定义特征，并且可能是哮喘中发现的高反应性和可变气流限制的原因。偏向于产生Th 2细胞因子的免疫应答被认为是哮喘气道炎症的发病机制。Th 2细胞因子白细胞介素9（IL-9）是在定位克隆和气道炎症动物模型基础上发现的一个哮喘候选基因。为了确定IL-9是否参与哮喘气道的慢性炎症，我们研究了IL-9和IL-9特异性受体链在哮喘气道中的表达，并与健康气道进行了比较。采用北方分析和逆转录聚合酶链反应技术，对9例健康人和7例轻度间歇性哮喘患者的气道上皮细胞和支气管肺泡灌洗液细胞中IL-9及其受体的表达进行了研究。还评价了原代和转化的人气道上皮细胞的体外IL-9特异性受体链表达。IL-9在健康或轻度哮喘个体的气道中未检测到。相比之下，在哮喘气道样本中发现了IL-9特异性受体链表达，但在健康对照中未发现。在体外，气道上皮细胞不表达IL-9特异性受体链，直到用干扰素γ刺激。提示IL-9可能在慢性气道炎症和哮喘的发病机制中起一定作用。
Inflammation of the airway wall is a defining feature in asthma and is likely the cause of the hyperreactivity and variable airflow limitation found in asthma. Immune response biased toward production of Th2 cytokines has been proposed as a mechanism in the pathogenesis of airway inflammation in asthma. The Th2 cytokine interleukin-9 (IL-9) is one candidate gene for asthma on the basis of position cloning and animal models of airway inflammation. To determine whether IL-9 is involved in the chronic inflammation of the asthmatic airway, we investigated the expression of IL-9 and the IL-9 specific receptor chain in asthmatic airways compared with healthy airways. IL-9 and IL-9 receptor expression in airway epithelial cells and bronchoalveolar lavage cells obtained at bronchoscopy of healthy (n = 9) and mild intermittent asthmatic individuals (n = 7) were studied by Northern analyses and reverse-transcription polymerase chain reaction technique. Primary and transformed human airway epithelial cells were also evaluated for IL-9 specific receptor chain expression in vitro. IL-9 was not detected in airways of healthy or mild asthmatic individuals. In contrast, IL-9 specific receptor chain expression was found in asthmatic airway samples but not in healthy controls. In vitro, airway epithelial cells did not express IL-9 specific receptor chain until stimulation with interferon gamma. Our results support that IL-9 may play a role in the mechanism leading to chronic airway inflammation and asthma.