The SEPALLATA-Like Gene OsMADS34 Is Required for Rice Inflorescence and Spikelet Development
The SEPALLATA-Like Gene OsMADS34 Is Required for Rice Inflorescence and Spikelet Development
复制标题
水稻花序和小穗发育需要 SEPALLATA 样基因 OsMADS34
DOI：
10.1104/pp.110.156711
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发表时间：
2010-06-01
期刊：
影响因子：
7.4
通讯作者：
Zhang, Dabing
中科院分区：
文献类型：
--
作者：
Gao, Xingchun;Liang, Wanqi;Zhang, Dabing
禾本科植物发育决定谷物产量的明显的花序和小穗。然而，在禾本科植物中花序和小穗的特化的潜在机制在很大程度上仍然未知。在此，我们报道了一个SEPALLATA（SEP）-like基因OsMADS 34在控制水稻花序和小穗发育中的生物学作用。OsMADS 34编码一个在酵母细胞中没有转录激活活性的MADS盒蛋白，该蛋白含有一个短的羧基末端。我们证明了OsMADS 34在根、叶、花序原基和小穗器官中的普遍表达。与野生型相比，osmads 34突变体的花序形态发生了改变，一次分枝数增加，二次分枝数减少。此外，osmads 34突变体表现出小穗数量减少和小穗形态改变，外稃/叶状伸长不育外稃。此外，双突变体osmads 34 osmads 1的分析表明，OsMADS 34指定的身份花器官，包括外稃/内稃，浆片，雄蕊和心皮，在另一个水稻SEP-样基因，OsMADS 1组合。总的来说，我们的研究表明，OsMADS 34和OsMADS 1的起源和多样化有助于不同的禾本科花序和小穗的起源。
Grass plants develop distinct inflorescences and spikelets that determine grain yields. However, the mechanisms underlying the specification of inflorescences and spikelets in grasses remain largely unknown. Here, we report the biological role of one SEPALLATA (SEP)-like gene, OsMADS34, in controlling the development of inflorescences and spikelets in rice (Oryza sativa). OsMADS34 encodes a MADS box protein containing a short carboxyl terminus without transcriptional activation activity in yeast cells. We demonstrate the ubiquitous expression of OsMADS34 in roots, leaves, and primordia of inflorescence and spikelet organs. Compared with the wild type, osmads34 mutants developed altered inflorescence morphology, with an increased number of primary branches and a decreased number of secondary branches. In addition, osmads34 mutants displayed a decreased spikelet number and altered spikelet morphology, with lemma/leaf-like elongated sterile lemmas. Moreover, analysis of the double mutant osmads34 osmads1 suggests that OsMADS34 specifies the identities of floral organs, including the lemma/palea, lodicules, stamens, and carpel, in combination with another rice SEP-like gene, OsMADS1. Collectively, our study suggests that the origin and diversification of OsMADS34 and OsMADS1 contribute to the origin of distinct grass inflorescences and spikelets.