Measurement of dimeric inhibin B throughout the human menstrual cycle
Measurement of dimeric inhibin B throughout the human menstrual cycle
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DOI：
10.1210/jc.81.4.1401
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发表时间：
1996-04-01
影响因子：
5.8
通讯作者：
McNeilly, AS
中科院分区：
文献类型：
--
作者：
Groome, NP;Illingworth, PJ;McNeilly, AS
本报告描述了一种特异性和敏感性的抑制素B测定方法的发展及其在测定人月经周期血浆中抑制素B浓度的应用。在双抗体酶联免疫吸附法中，将针对β - b亚基合成肽的单克隆抗体与针对抑制素α -亚基序列的抗体结合。经验证的检测限为10 pg/mL，与抑制素a的交叉反应性为0.5%。通过这种免疫分析，我们发现抑制素B的血浆浓度在卵泡期早期迅速上升，在周期FSH上升后的第二天达到85.2 +/- 9.6 pg/mL的峰值，然后在卵泡期的其余时间逐渐下降。中期LH峰后2天抑制素B浓度出现短暂高峰（133.6 +/- 31.2 pg/mL），随后降至较低浓度(
This report describes the development of a specific and sensitive assay for inhibin B and its application to the measurement of inhibin B concentrations in plasma during the human menstrual cycle. A monoclonal antibody raised against a synthetic peptide from the beta B-subunit was combined with an antibody to an inhibin alpha-subunit sequence in a double antibody enzyme-linked immunosorbent assay format. The validated assay had a limit of detection of 10 pg/mL and 0.5% cross-reactivity with inhibin A.Using this immunoassay, we found that the plasma concentration of inhibin B rose rapidly in the early follicular phase to a peak of 85.2 +/- 9.6 pg/mL on the day after the intercycle FSH rise, then fell progressively during the remainder of the follicular phase. Two days after the midcycle LH peak, there was a short lived peak in the inhibin B concentration (133.6 +/- 31.2 pg/mL), which then fell to a low concentration (