Applicability of laser-induced Raman microscopy for in situ monitoring of imine formation in a glass microfluidic chip
Applicability of laser-induced Raman microscopy for in situ monitoring of imine formation in a glass microfluidic chip
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DOI：
10.1002/jrs.1038
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发表时间：
2003-10-01
影响因子：
2.5
通讯作者：
Lee, EK
中科院分区：
文献类型：
--
作者：
Lee, M;Lee, JP;Lee, EK
激光诱导拉曼显微镜已被用来说明其适用性，在玻璃微流控芯片中的亚胺形成反应的原位监测。为了监测微通道中的扩散过程，在2.7穆尔min（-1）的恒定流速下，在沿着通道的各个点处测量拉曼光谱。还在没有流动的情况下测量时间依赖性拉曼光谱，以监测拉曼峰到完全转化的变化。成功地监测到反应物苯甲醛在1700 cm（-1）处C=O伸缩峰的消失和产物亚胺在1628 cm（-1）处的拉曼峰的出现。此外，在1550-1630 cm（-1）范围内还观察到三个苯基伸缩模的强度增加。该振动模式的拉曼强度的增加是由通过产物的-C=N -桥连基团的两个苯环之间的有效π电子共轭引起的。激光诱导拉曼显微镜使我们能够监测原位产品的形成，并获得详细的结构信息，在玻璃微流控芯片。版权所有（C）2003约翰威利父子有限公司。
Laser-induced Raman microscopy has been used to illustrate its applicability for the in situ monitoring of imine formation reaction in a glass microfluidic chip. In order to monitor the diffusion process in a micro channel, the Raman spectra were measured at various points along the channel with a constant flow rate of 2.7 mul min(-1). Time-dependent Raman spectra were also measured without flow in order to monitor the variation of Raman peaks to a complete conversion. The disappearance of the C=O stretching peak at 1700 cm(-1) of the reactant, benzaldehyde, and the appearance of the Raman peak for the product, an imine, at 1628 cm(-1) were successfully monitored. In addition, the intensity increases of three phenyl stretching modes in the 1550-1630 cm(-1) region were also observed. The increase in Raman intensity for this vibrational mode is caused by an effective pi-electron conjugation between two phenyl rings through the -C=N - bridging group of the product. Laser-induced Raman microscopy enables us to monitor in situ product formation and to obtain detailed structural information in a glass microfluidic chip. Copyright (C) 2003 John Wiley Sons, Ltd.