Effect of the cell type and cell density on the binding of living cells to a silica particle: An atomic force microscope study
Effect of the cell type and cell density on the binding of living cells to a silica particle: An atomic force microscope study
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DOI：
10.1016/j.colsurfb.2006.09.020
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发表时间：
2006-12-01
影响因子：
5.8
通讯作者：
Higashitani, Ko
中科院分区：
文献类型：
--
作者：
Pyo, Nayoung;Tanaka, Saaya;Higashitani, Ko
我们利用原子力显微镜研究了细胞类型和细胞密度如何影响细胞与模型药物递送系统（DDS）载体纳米颗粒之间的粘附。我们使用了三种不同的锚定依赖性细胞，即活小鼠成纤维细胞（1929），活人类结肠癌细胞（Caco2）和活小鼠恶性黑色素瘤细胞（1316F10）。对于DDS模型纳米粒子，我们使用了二氧化硅胶体。为了将黏附力与细胞类型联系起来，用血细胞计测定细胞的生长曲线。光镜下观察细胞在不同阶段的形态，原子力显微镜下观察细胞表面形貌。力测量表明，无论电池密度如何，Caco2电池与二氧化硅颗粒的结合都很小。L929电池结合良好的二氧化硅颗粒低和高的电池密度。B16F10电池在低电池密度时与二氧化硅颗粒结合很少，但在高电池密度时结合良好。AFM图像显示L929细胞不含褶皱。然而，B16F10细胞在低细胞密度时，细胞表面出现褶皱，而在高细胞密度时，细胞表面没有褶皱。由于文献也报道了Caco2细胞含有褶皱，这些结果表明，有褶皱的细胞比没有褶皱的细胞对二氧化硅颗粒的粘附更少。细胞中褶皱的存在可能减少了细胞上可以与硅烷醇基团形成氢键或范德华键的位点的数量。(c) 2006 Elsevier b.v.版权所有
We used the atomic force microscope to study how the cell type and the density of cells adsorbed at a substrate can affect the adhesion between a living cell and a model drug delivery system (DDS) carrier nano-particle. We used three different anchorage-dependent cells, i.e., a living mouse fibroblast cell (1929), a living human colon cancer cell (Caco2), and a living mouse malignant melanoma cell (1316F10). For the DDS model nano-particle, we used a silica colloid. In order to correlate the adhesion force with the cell types, the growth curve of the cells were determined with a haemocytometer. The shapes of the cells at the different stages were monitored by light microscopy, and the morphology of their surfaces obtained by tapping mode atomic force microscopy.Force measurements showed that the Caco2 cell bound little to a silica particle, regardless of the cell density. The L929 cell bound well to a silica particle for low and high cell densities. The B16F10 cell bound little to a silica particle for low cell densities, but bound well for high cell densities. AFM images showed that the L929 cell did not contain folds. The B16F10 cells, however, displayed folds in the cell surface for low cell densities, but no folds in the cell for high cell densities. As literature also reported that the Caco2 cell contains folds, these results suggested that cells with folds showed less adhesion to a silica particle than cells without folds. The presence of folds in the cell presumably decreased the number of sites on the cell that could hydrogen bond or undergo van der Waals binding with the silanol groups of the silica particle. (c) 2006 Elsevier B.V All rights reserved.