Rapid and sensitive in-situ detection of polar antibiotics in water using a disposable Ag-graphene sensor based on electrophoretic preconcentration and surface-enhanced Raman spectroscopy
Rapid and sensitive in-situ detection of polar antibiotics in water using a disposable Ag-graphene sensor based on electrophoretic preconcentration and surface-enhanced Raman spectroscopy
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使用基于电泳预浓缩和表面增强拉曼光谱的一次性银石墨烯传感器快速、灵敏地原位检测水中的极性抗生素
DOI：
10.1016/j.bios.2012.12.005
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发表时间：
2013-05-15
影响因子：
12.6
通讯作者：
Long, Yi-Tao
中科院分区：
文献类型：
--
作者：
Li, Yuan-Ting;Qu, Lu-Lu;Long, Yi-Tao
开发了一种一次性银 - 石墨烯传感器，用于利用电泳预富集（EP）和表面增强拉曼光谱（SERS）对水中的极性抗生素进行快速、灵敏的原位检测。通过将一种简便的一锅法合成的银 - 石墨烯纳米复合材料沉积在一次性丝网印刷电极（SPEs）上制备了银 - 石墨烯传感器，并通过透射电子显微镜（TEM）和原子力显微镜（AFM）对其进行了表征。对所开发传感器的SERS特性和检测适用性进行了系统研究。结果表明，在EP过程中施加不同电位后，极性抗生素可选择性地吸附在带相反电荷的传感器上，并且通过适当的预富集时间可以实现抗生素SERS信号的有效放大。此外，银 - 石墨烯传感器可通过石墨烯与抗生素之间的弱π - π相互作用促进分子吸附，进一步提高SERS检测的灵敏度。在最佳EP条件下，可在10分钟内获得含有四种不同抗生素的混合溶液的代表性SERS光谱，并且每种抗生素都可通过其特征峰轻松区分，检测水平可达亚纳摩尔级。结果表明，所提出的基于EP - SERS的一次性银 - 石墨烯传感器可用于在无需预分离步骤的情况下对水样中的极性抗生素进行快速、灵敏的原位检测。（c）2012爱思唯尔公司。版权所有。
A disposable Ag-graphene sensor was developed for rapid and sensitive in-situ detection of polar antibiotics in water using electrophoretic preconcentration (EP) and surface-enhanced Raman spectroscopy (SERS). The Ag-graphene sensor was fabricated by depositing Ag-graphene nanocomposites synthesized through a facile one-pot method on the disposable screen-printed electrodes (SPEs) and characterized by transmission electron microscopy (TEM) and atomic force microscopy (AFM). SERS properties and detection applicability of the developed sensor were systematically investigated. It is shown that the polar antibiotics can be selectively adsorbed on the oppositely charged sensors after applying different potentials during the EP procedure, and the SERS signals of antibiotics with an effective amplification can be achieved with proper time of preconcentration. Moreover, the Ag-graphene sensor could facilitate the molecule adsorption through weak pi-pi interactions between graphene and antibiotics, further improving the sensitivity of SERS detection. Under the optimum EP conditions, the representative SEAS spectra of a mixed solution containing four different antibiotics can be obtained within 10 min, and each antibiotic is easily distinguished by its characteristic peaks with a sub-nM detection level. The results demonstrate that the proposed disposable Ag-graphene sensor based on EP-SERS can be used for rapid and sensitive in-situ detection of polar antibiotics in aqueous samples without a pre-separation step. (c) 2012 Elsevier B.V. All rights reserved.