Colloidal Stability of Silk Fibroin Nanoparticles Coated with Cationic Polymer for Effective Drug Delivery
Colloidal Stability of Silk Fibroin Nanoparticles Coated with Cationic Polymer for Effective Drug Delivery
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DOI：
10.1021/acsami.5b05335
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发表时间：
2015-09-30
影响因子：
9.5
通讯作者：
Shao, Zhengzhong
中科院分区：
文献类型：
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作者：
Wang, Suhang;Xu, Tao;Shao, Zhengzhong
通常，丝素蛋白纳米颗粒（SFNPs）是体内递送药物或其他生物活性物质的理想候选者。然而，它们的进一步应用在很大程度上受到SENP的低胶体稳定性的限制，因为它们倾向于在生物介质中聚集。为了解决这个问题，SFNP复合材料与核壳结构（CS-SFNPs），通过涂覆SFNPs与四个不同的选择的阳离子聚合物，乙二醇壳聚糖，N，N，N-三甲基壳聚糖，聚乙烯亚胺，聚乙二醇化聚乙烯亚胺，通过静电相互作用。根据DLS和NTA结果，与裸SFNPs相比，CS-SFNPs在生物介质中显示出更高的胶体稳定性。当用人宫颈癌（HeLa）细胞处理时，CS-SFNPs被有效地内化并在溶酶体中积累;并且当负载抗癌药物DOX时，CS-SFNPs也显示出对HeLa细胞的更高的细胞毒性。我们的研究结果表明，所制造的CS-SFNPs在生物介质中具有理想的胶体稳定性，有可能被用作抗癌药物递送系统的药物载体。
Generally, silk fibroin nanoparticles (SFNPs) are great candidates to deliver drugs or other bioactive substances in vivo. However, their further applications are largely limited by the low colloidal stability of SENPs, as they tend to aggregate in biological media. To address this issue, SFNP composite materials with a core shell structure (CS-SFNPs) were fabricated by coating SFNPs with four different selected cationic polymers, glycol chitosan, N,N,N-trimethyl chitosan, polyethylenimine, and PEGylated polyethylenimine, through electrostatic interaction. According to the DLS and NTA results, compared with the bare SFNPs, the CS-SFNPs showed much higher colloidal stability in biological media. When treated with human cervical carcinoma (HeLa) cells, the CS-SFNPs were efficiently internalized and accumulated in lysosome; and when loaded with an anticancer drug, DOX, the CS-SFNPs also showed higher cytotoxicity against HeLa cells. Our results suggest that the fabricated CS-SFNPs with desirable colloidal stability in biological media have the potential to be employed as drug carriers for the anticancer drug delivery system.