Variation with embryonic development and regional localization of specific [3H]phorbol 12,13-dibutyrate binding to brain.
Variation with embryonic development and regional localization of specific [3H]phorbol 12,13-dibutyrate binding to brain.
复制标题
特定[3H]佛波醇12,13-二丁酸酯与大脑结合的胚胎发育和区域定位的变化。
作者：
D. S. Nagle;S. Jaken;M. Castagna;P. Blumberg
我们以前的特点是特异性结合的佛波酯肿瘤促进剂[20- 3 H]佛波12，13-二丁酸几种组织，包括小鼠皮肤和大脑。我们在这里报告，在鸡脑中的特异性结合活性在发育过程中显着增加。在整个胚胎鸡脑，结合增加2.0至6.8至10.6 pmol/mg蛋白在7，14和20天，分别。成年鸡脑结合15 pmol/mg蛋白质。与特异性结合活性相反，结合亲和力保持恒定。牛脑的代表值范围为4.6 pmol/mg（髓质）至38.1 pmol/mg（额叶）。结合亲和力没有变化。检查了假定的神经递质和拮抗剂对结合的抑制。D-普萘洛尔和奎尼丁，膜稳定药物，抑制竞争性结合，虽然在mM浓度。
We have previously characterized specific binding of the phorbol ester tumor promoter [20-3H]phorbol 12,13-dibutyrate to several tissues, including mouse skin and brain. We report here that specific binding activity in chicken brain increases dramatically during development. In whole embryonic chicken brain, binding increased from 2.0 to 6.8 to 10.6 pmol/mg of protein at 7, 14, and 20 days, respectively. Adult chicken brain bound 15 pmol/mg of protein. In contrast to specific binding activity, binding affinity remained constant. Substantial regional localization of binding activity within the brain was found. For calf brain, representative values ranged from 4.6 pmol/mg for medulla to 38.1 pmol/mg for frontal lobe. Binding affinity did not vary. Inhibition of binding by postulated neurotransmitters and antagonists was examined. D-Propranolol and quinidine, membrane-stabilizing drugs, inhibited binding competitively, although at mM concentrations.