Electron microprobe analysis of metal ion transport in cortical bone.
Electron microprobe analysis of metal ion transport in cortical bone.
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皮质骨中金属离子传输的电子微探针分析。
DOI：
10.1002/jbm.820180412
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Galante,J
中科院分区：
文献类型：
--
作者：
Woodman,J;Shinn,W;Urban,R;Galante,J
材料与方法本实验选用三种金属：钴铬合金（ASTM F75-76）、6多相35%镍（ASRVI F% 2-78）和纪念纯钛（ASTM F67-77）。*成年杂种狗植入长8.9 mm，直径4.4 mm的实体标本。一个标本放置于髁上区，另一个标本放置于股骨骨干区，与骨长轴垂直。这一过程在9只狗的双侧进行，3只狗的金属测试。实验周期为2年。献祭后，植入物和周围的骨头被取出。在6%的戊二醛中固定12小时，然后冲洗并包埋在环氧树脂中。然后将样品块切成两半，表面用金刚石抛光至1.0 p~。植入物和移除植入物的切片都被碳涂层，使其导电，用于电子探针分析。采用ARL EMX-SM微探针分析仪和三个晶体光谱仪。在每个样品的几个区域收集微探针数据作为计数与距离的关系，以确定种植体附近金属（钛、镍、钴或铬）的浓度梯度。纯形式的所有四种金属都用作标准，并对密度较低的介质进行适当的校正。此外，还测定了钙磷比，以证明采样区域实际上是成熟的骨骼。
MATERIALS AND METHODSThree metals were chosen for this experiment: cobalt-chromium alloy (ASTM F75-76), 6 Multiphase'35% Nickel (ASRVI F% 2-78),'and commemdy pure titanium (ASTM F67-77).* Adult mongrel dogs were implanted with solid specimens 8.9 mm in length by 4.4 mm in diameter. One specimen was placed in the supracondylar region and a second specimen was placed in the diaphyseal region of the femur, perpendicular to the long axis of the bone. This procedure was done bilaterally in nine dogs, three dogs-metal tested.One experimental time period of 2 years was chosen. After sacrifice, the implants with the surrounding bone were retrieved. They were fixed in 6% gluteraldehyde for 12 h, then rinsed and embedded in expoxy resin. The sample block was then cut in half and the surface polished to 1.0 p~ with diamond. Sections both with the implant and with the implant removed were carbon coated to make them electrically conductive for electron microprobe analysis. An ARL EMX-SM microprobe analyzer with three crystal spectrometers was used. Microprobe data as counts versus distance was collected at several areas in each sample to determine the concentration gradient of metal (titanium, nickel, cobalt, or chromium) in the vicinity of the implant. All four metals in the pure form were used as a standard with appropriate correction for a lower density medium. Additionally caldumphosphorus ratios were determined to venfy the sampling area was in fact mature bone.