Overlapping and differential expression of BIG-2, BIG-1, TAG-1, and F3: four members of an axon-associated cell adhesion molecule subgroup of the immunoglobulin superfamily.
Overlapping and differential expression of BIG-2, BIG-1, TAG-1, and F3: four members of an axon-associated cell adhesion molecule subgroup of the immunoglobulin superfamily.
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BIG-2、BIG-1、TAG-1 和 F3 的重叠和差异表达：免疫球蛋白超家族轴突相关细胞粘附分子亚组的四个成员。
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发表时间：
1995
期刊：
影响因子：
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通讯作者：
K. Mori
中科院分区：
文献类型：
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作者：
Y. Yoshihara;M. Kawasaki;A. Tamada;S. Nagata;H. Kagamiyama;K. Mori
轴突相关细胞粘附分子（Axon-associated cell adhesion molecules, AxCAMs）在功能性神经网络的形成、维持和可塑性中起着至关重要的作用。我们在这里报道了一种新的AxCAM， BIG-2的分子克隆。BIG-2是免疫球蛋白（Ig）超家族TAG-1/F3亚群的成员，具有6个Ig样结构域，4个纤维连接蛋白iii型重复序列和一个糖基磷脂酰肌醇锚定结构域。重组BIG-2蛋白在体外作为神经元底物时具有促进神经突生长的活性。为了比较BIG-2与其他TAG-1/F3亚群成员的空间表达模式，我们使用BIG-2、BIG-1、TAG-1和F3特异性的核探针对成年和发育中的大鼠脑切片进行了原位杂交分析。四个AxCAM转录本显示出细胞类型特异性表达模式，具有重叠和不同的特征。例如，在成人海马中，我们观察到BIG-1 mRNA在齿状回颗粒细胞中特异性表达，BIG-2 mRNA在CA1锥体细胞中高度表达，TAG-1 mRNA主要在CA3锥体细胞中表达，而F3 mRNA在所有这些区域的神经元中都有表达。这些结果表明，BIG-2、BIG-1、TAG-1和F3可能在大脑特定神经元网络的形成和维持中发挥重要作用。
Axon-associated cell adhesion molecules (AxCAMs) play crucial roles in the formation, maintenance, and plasticity of functional neuronal networks. We report here a molecular cloning of a novel AxCAM, BIG-2. BIG-2 is a member of TAG-1/F3 subgroup of the immunoglobulin (Ig) superfamily, with six Ig-like domains, four fibronectin type III-like repeats, and a glycosyl phosphatidylinositol-anchoring domain. Recombinant BIG-2 protein had a neurite outgrowth-promoting activity when used as a substrate for neurons in vitro. To survey the spatial expression pattern of BIG-2 in comparison with other TAG-1/F3 subgroup members, an in situ hybridization analysis was performed in adult and developing rat brain sections with riboprobes specific for BIG-2, BIG-1, TAG-1, and F3. The four AxCAM transcripts displayed cell type-specific expression patterns with overlapping and distinct profiles. In adult hippocampus, for example, we observed BIG-1 mRNA specifically in granule cells of the dentate gyrus, BIG-2 mRNA highly in the CA1 pyramidal cells, TAG-1 mRNA predominantly in the CA3 pyramidal cells, and F3 mRNA in neurons in all of these fields. These results suggest that BIG-2, BIG-1, TAG-1, and F3 may play important roles in the formation and maintenance of specific neuronal networks in the brain.