Mucins (MUC1 and MUC3) of gastrointestinal and breast epithelia reveal different and heterogeneous tumor-associated aberrations in glycosylation
Mucins (MUC1 and MUC3) of gastrointestinal and breast epithelia reveal different and heterogeneous tumor-associated aberrations in glycosylation
复制标题
DOI：
10.1177/002215549704501111
复制
发表时间：
1997-11-01
影响因子：
3.2
通讯作者：
Karsten, U
中科院分区：
文献类型：
--
作者：
Cao, Y;Blohm, D;Karsten, U
在一项全面的研究中，我们分别检测了正常和肿瘤胃肠道和乳腺上皮中膜和分泌黏液MUC1和MUC3的表达，在神经氨酸酶、a-聚焦酶或碳水化合物特异性高酸盐氧化特异性改变其糖结构之前和之后。通过原位杂交，MUC1 mRNA也在正常结肠组织中被鉴定出来。数据显示，正常的结直肠上皮细胞表达MUC1 mKNA和蛋白，经过高酸盐氧化后，所有检测的MUC1特异性抗体都可以检测到。在结肠肿瘤发生过程中，MUC1在没有高碘酸盐治疗的情况下被识别出来，并伴有高度发育不良的病变和恶性状态。在乳腺中，MUC1在正常上皮和癌中可被大多数抗体检测到，高碘酸盐预处理可增强MUC1的染色，酶处理可增强MUC1的染色。MUC3在正常和肿瘤结直肠组织中均可检测到，在高碘酸氧化后染色更强烈。它在正常乳腺中不存在，但在20例乳腺癌中有7例表达。因此，粘蛋白糖基化不完全、分布异常、异位表达是恶性肿瘤的特征。高碘酸氧化可广泛应用于免疫组织化学，用于检查糖基化的变化和检测被聚糖掩盖的抗原。
In a comprehensive study, we examined the expression of the membrane and secretory mucins MUC1 and MUC3, respectively, in normal and neoplastic gastrointestinal and breast epithelia before and after specific alterations of their glycan structures by neuraminidase, a-fucosidase, or carbohydrate-specific periodate oxidation. MUC1 mRNA was also identified in normal colorectal tissues by in situ hybridization. The data revealed that normal colorectal epithelia express both MUC1 mKNA and protein, which were detectable after periodate oxidation with all tested MUC1-specific antibodies. During tumorigenesis in the colon, MUC1 became recognizable without periodate treatment concomitantly with highly dysplastic lesions and the malignant state. In the breast, in which MUC1 is detectable with most antibodies in normal epithelium as well as in carcinomas, staining could be enhanced by pretreatment with periodate and casually by enzyme treatments. MUC3 was detectable in normal and neoplastic colorectal tissues and was more intensely stained after periodate oxidation. It was absent in normal breast even after pretreatment but was expressed in seven of 20 breast carcinomas. Therefore, incomplete glycosylation, abnormal distribution, and ectopic expression of mucins are characteristics of malignancy. Periodate oxidation may be widely applicable to immunohistochemistry for examining changes in glycosylation and for detecting antigens masked by glycans.