Novel and traditional traits of frozen-thawed porcine sperm related to in vitro fertilization success
Novel and traditional traits of frozen-thawed porcine sperm related to in vitro fertilization success
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DOI：
10.1016/j.theriogenology.2014.04.006
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发表时间：
2014-07-15
期刊：
影响因子：
2.8
通讯作者：
Miller, David J.
中科院分区：
文献类型：
--
作者：
Daigneault, Bradford W.;McNamara, Kelli A.;Miller, David J.
冷冻保存的精液允许使用单次射精进行重复分析，通过消除新鲜精液观察到的射精间变异性，有可能提高体外受精的一致性。然而，冷冻和解冻过程会导致精子功能和体外受精成功率受损。精液样本在用于体外受精之前通常会进行活力筛查。低于指定运动阈值的样本可以被丢弃。我们的目标是确定解冻后的精子活力、可能指示精子功能的其他特征或输卵管结合的新测定是否与 IVF 成功相关。将 16 头公猪的精液冷却至 15 摄氏度，过夜运输，然后冷冻保存。解冻精液并在显微镜下记录活力并使用计算机自动精子评估进行确认。每个样本均通过 IVF 进行两到三次独立重复测试。采用回归和相关分析来确定精子性状之间的相互关系以及解冻后活力、精子-输卵管结合和体外受精结果之间的关系。在检查的精子性状中，精子顶体完整性与解冻后活力呈负相关（r(2) = 0.64），但与 IVF 结果无关。与输卵管聚集体结合的精子数量与 IVF 多精率相关（r(2) = 0.62，P < 0.05），但与总体 IVF 率相关性较小（r(2) = 0.31，P > 0.10）。解冻后活力与 IVF 单精子受精存在一定关系（P = 0.06，r(2) = 0.08），但与其他 IVF 结果无关。我们的结果表明，冻融公猪精子的解冻后活力与顶体完整性密切相关，但在预测 IVF 成功方面的用途有限。与输卵管细胞结合的精子数量与 IVF 多精率相关，并且可能比传统的精子活力和顶体状态评估更能指示体外精子功能。 (C) 2014 Elsevier Inc. 保留所有权利。
Cryopreserved semen allows the use of single ejaculates for repeated analyses, potentially improving IVF consistency by eliminating interejaculate variability observed with fresh semen. However, the freezing and thawing processes result in compromised sperm function and IVF success. Semen samples are often screened for motility before use for IVF. Samples that are below a designated motility threshold may be discarded. Our objectives were to determine if post-thaw sperm motility, other traits that may be indicative of sperm function, or a novel assay of oviduct binding were related to IVF success. Semen from 16 boars was cooled to 15 degrees C for overnight shipment before cryopreservation. Semen was thawed and motility was recorded microscopically and confirmed using computer-automated sperm assessment. Each sample was tested by IVF in two to three independent replicates. Regression and correlation analyses were employed to determine the interrelationships between sperm traits and the relationships between post-thaw motility, sperm-oviduct binding and IVF outcomes. Among the sperm traits examined, sperm acrosome integrity was negatively correlated with post-thaw motility (r(2) = 0.64) but not with IVF results. The number of sperm bound to oviduct aggregates was correlated with IVF polyspermy rates (r(2) = 0.62, P < 0.05) but less with overall IVF rates (r(2) = 0.31, P > 0.10). There was some relationship of post-thaw motility with IVF monospermic fertilization (P = 0.06, r(2) = 0.08) but not to other IVF outcomes. Our results indicate that post-thaw motility of frozen-thawed boar sperm is strongly related to acrosome integrity but has limited use for predicting IVF success. The number of sperm bound to oviduct cells was related to IVF polyspermy rates and may be more indicative of in vitro sperm function than traditional sperm motility and acrosome status evaluation. (C) 2014 Elsevier Inc. All rights reserved.