Genetic variation in Symbiodinium (=Gymnodinium) microadriaticum Freudenthal, and specificity in its symbiosis with marine invertebrates. I. Isoenzyme and soluble protein patterns of axenic cultures of Symbiodinium microadriaticum
Genetic variation in Symbiodinium (=Gymnodinium) microadriaticum Freudenthal, and specificity in its symbiosis with marine invertebrates. I. Isoenzyme and soluble protein patterns of axenic cultures of Symbiodinium microadriaticum
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Symbiodinium (=Gymnodinium) microadriaticum Freudenthal 的遗传变异，及其与海洋无脊椎动物共生的特异性。
DOI：
10.1098/rspb.1980.0031
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
R. Trench
中科院分区：
文献类型：
--
作者：
D. A. Schoenberg;R. Trench
从两门17个种的40个宿主个体（或菌落）中分离到12个菌株，每个菌株都具有4种同工酶的独特组合。每一个单独的主机似乎包含一个藻类的寄生均匀的人口。在交叉感染菌株的重新分离后，每种特征同工酶模式得以保持（i. e.已通过实验将菌株引入到不同于最初分离菌株的物种的宿主中），表明在每个菌株中观察到的蛋白质模式的遗传基础。通过计算相似系数，用未加权平均连锁聚类法构建系统树，可将供试菌株分为3个类群。从藻类获得的可溶性蛋白质的模式也strainspecific。通过聚丙烯酰胺凝胶圆盘电泳解析的蛋白质模式分析获得的数据构建的树状图是相似的，从同工酶模式的数据构建的树状图，但建议菌株之间的“表型”距离大于所示的同工酶模式。
Axenically culturedSymbiodinium (=Gymnodinium) microadriaticum Freudenthal, isolated from 40 host individuals (or colonies) representing 17 species in two phyla were resolved into 12 strains, each strain having a unique combination of four isoenzyme patterns as revealed by starch gel electrophoresis. Each individual host appeared to contain an electrophoretically homogeneous population of algae. Each characteristic isoenzyme pattern was maintained after re-isolation of a cross-infecting strain (i. e. a strain that had been experimentally introduced into a host of a species other than that from which it was originally isolated), indicating a genetic basis for the protein patterns observed in each strain. The strains could be separated into three groups after constructing a dendrogram by the unweighted average linkage clustering method based on calculations of similarity coefficients. Soluble protein patterns obtained from the algae were also strainspecific. A dendrogram constructed from data obtained by analysis of protein patterns resolved by a polyacrylamide gel disc electrophoresis was similar to the dendrogram constructed from the data on isoenzyme patterns, but suggested that the ‘phenetic’ distances between strains were larger than indicated by the isoenzyme patterns.