Differential effects of baclofen on sustained and transient cells in the mudpuppy retina.
Differential effects of baclofen on sustained and transient cells in the mudpuppy retina.
复制标题
巴氯芬对泥犬视网膜中持续和瞬时细胞的不同影响。
DOI：
10.1152/jn.1989.61.2.374
复制
发表时间：
1989
影响因子：
2.5
通讯作者：
Bai,SH
中科院分区：
文献类型：
--
作者：
Slaughter,MM;Bai,SH
1.巴氯芬，一种γ-氨基丁酸（GABA）/B受体激动剂，在记录mudpuppy视网膜中的二级和三级神经元的反应时被应用。巴氯芬受体主要局限于无长突细胞和神经节细胞。2.巴氯芬超极化膜电位的许多，但不是全部，三级神经元。这涉及输入电导的增加，可能与钾通道的开放有关。3.与GABA/B受体激活相关的输入电导的最大增加约为GABA/A受体激活产生的输入电导的三分之一。4.巴氯芬抑制持续反应，但增强瞬时反应。净效应是在巴氯芬存在下，整个内层视网膜的反应变得更加短暂。5.在持续的细胞巴氯芬不仅抑制持续的反应，但也发现大的瞬态反应。因此，巴氯芬将这些细胞的光反应从持续性转变为短暂性。这表明，持续的细胞收到显着的瞬态兴奋，这通常是由持续的输入掩盖。6. GABA/B受体在控制无长突细胞和神经节细胞的反应特性和信息内容的作用进行了讨论。
1. Baclofen, a gamma-aminobutyric acid (GABA)/B receptor agonist, was bath applied while recording the responses of second- and third-order neurons in the mudpuppy retina. Baclofen receptors were largely restricted to amacrine and ganglion cells. 2. Baclofen hyperpolarized the membrane potential of many, but not all, third-order neurons. This involved an increase in input conductance, probably associated with an opening of potassium channels. 3. The maximal increase in input conductance associated with the activation of GABA/B receptors was approximately one-third of that produced by activation of GABA/A receptors. 4. Baclofen suppressed sustained responses but enhanced transient responses. The net effect was that responses throughout the inner retina became more transient in the presence of baclofen. 5. In sustained cells baclofen not only suppressed the sustained responses but also revealed large transient responses. Thus baclofen converted the light responses of these cells from sustained to transient. This suggests that sustained cells receive significant transient excitation which is normally masked by the sustained inputs. 6. The role of the GABA/B receptor in controlling response characteristics and information content of amacrine and ganglion cells is discussed.