Preclinical validation of the new vitrification device possessing a feature of absorbing excess vitrification solution for the cryopreservation of human embryos
Preclinical validation of the new vitrification device possessing a feature of absorbing excess vitrification solution for the cryopreservation of human embryos
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DOI：
10.1111/jog.14176
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发表时间：
2020-01-10
影响因子：
1.6
通讯作者：
Osuga, Yutaka
中科院分区：
文献类型：
--
作者：
Takahashi, Nozomi;Harada, Miyuki;Osuga, Yutaka
目的胚胎冷冻保存是辅助生殖技术的重要组成部分。本研究的目的是检查一种新的玻璃化冷冻装置，北里玻璃化冷冻系统（KVS），在人类胚胎冷冻保存的有效性和易用性。方法采用KVS和Cryotop（对照）玻璃化冷冻系统对卵裂期和囊胚期的人胚胎进行玻璃化冷冻和复温。对卵裂期和囊胚期胚胎的存活率以及卵裂期胚胎的发育能力进行了评价。四个人在玻璃化冷冻和升温胚胎经验不足，测试了KVS和Cryotop。观察胚胎玻璃化时间和脱落时间。结果在卵裂期，KVS和Cryotop的胚胎存活率和囊胚发育率无显著差异（分别为100vs96.8%和63.3vs61.3%）。在囊胚期，KVS和Cryotop之间的再扩张率没有显著差异（100 vs 88.9%）。KVS的玻璃化时间比Cryotop短。KVS和Cryotop之间的解脱时间无显著差异。结论北里玻璃化冷冻系统操作简单，即使是经验不足的使用者也能操作，KVS冷冻人胚胎的存活率与Cryotop相当。
Aim The cryopreservation of embryos is essential for assisted reproductive technology field. The aim of the present study is to examine the efficacy and ease of use of a new vitrification device, Kitasato Vitrification System (KVS), in cryopreservation of human embryos. Methods Human embryos at the cleavage or blastocyst stage were vitrified and warmed by KVS or Cryotop (control device). The survival of cleavage- and blastocyst-stage embryos and the developmental competence of cleavage-stage embryos were evaluated. Four individuals inexperienced in vitrification and warming embryos tested both KVS and Cryotop. The vitrification time and the detachment time of the embryos were evaluated. Results At the cleavage stage, there were no significant differences in the survival rate and the development rate to the blastocyst stage between KVS and Cryotop (100 vs 96.8% and 63.3 vs 61.3%, respectively). At the blastocyst stage, there was no significant difference in the re-expansion rate between KVS and Cryotop (100 vs 88.9%). The vitrification time was shorter for KVS than Cryotop. There was no significant difference in the detachment time between KVS and Cryotop. Conclusion Kitasato Vitrification System is easy to operate, even for inexperienced users, and the viability of human embryos vitrified by KVS is comparable to that of Cryotop, a widely used vitrification device.