Efficiency of protection of guinea pigs against infection with Bacillus anthracis spores by passive immunization
Efficiency of protection of guinea pigs against infection with Bacillus anthracis spores by passive immunization
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DOI：
10.1128/iai.70.2.544-550.2002
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发表时间：
2002-02-01
影响因子：
3.1
通讯作者：
Altboum, Z
中科院分区：
文献类型：
--
作者：
Kobiler, D;Gozes, Y;Altboum, Z
评价被动免疫作为治疗鼻内感染炭疽芽孢杆菌孢子的豚鼠的暴露后预防措施的有效性。针对致死毒素组分（PA或LF）或针对产毒素菌株（Sterne）的抗血清用于该评价。所有抗血清均表现出针对相应抗原的高酶联免疫吸附测定滴度，体外小鼠巨噬细胞系（J774A.1）中细胞毒性活性的高中和滴度，以及在用致死毒素攻击前或与致死毒素孵育后直接给予Fisher大鼠时的体内毒性中和。在这些试验中，抗LF抗血清表现出最高的中和效率，其次是抗Sterne和抗PA。时间依赖性和抗体剂量所需的给予后exasterative保护的各种抗体感染的豚鼠25 - 50%致死剂量的卷孢子进行了检查。兔抗PA血清是最有效的。腹腔注射抗PA血清24 111感染后保护90%的感染动物，而抗Sterne和抗LF是不太有效的。这些结果进一步强调了抗PA抗体在赋予针对B的保护中的重要性。炭疽感染，并证明了这种抗体作为有效的抗炭疽病的暴露后治疗有效应用的能力。
The efficacy of passive immunization as a postexposure prophylactic measure for treatment of guinea pigs intranasally infected with Bacillus anthracis spores was evaluated. Antisera directed either against the lethal toxin components (PA or LF) or against a toxinogenic strain (Sterne) were used for this evaluation. All antisera exhibited high enzyme-linked immunosorbent assay titers against the corresponding antigens, high titers of neutralization of cytotoxicity activity in an in vitro mouse macrophages cell line (J774A.1), as well as in vivo neutralization of toxicity when administered either directly to Fisher rats prior to challenge with the lethal toxin or after incubation with the lethal toxin. In these tests, anti-LF antiserum exhibited the highest neutralization efficiency, followed by anti-Sterne and anti-PA. The time dependence and antibody dose necessary for conferring postexposure protection by the various antibodies of guinea pigs infected with 25 50% lethal doses of Vollum spores was examined. Rabbit anti-PA serum was found to be the most effective. Intraperitoneal injections of anti-PA serum given 24 111 postinfection protected 90% of the infected animals, whereas anti-Sterne and anti-LF were less effective. These results further emphasizes the importance of anti-PA antibodies in conferring protection against B. anthracis infection and demonstrated the ability of such antibodies to be effectively applied as an efficient postexposure treatment against anthrax disease.