STIMULATION OF LIVER GROWTH BY EXOGENOUS HUMAN HEPATOCYTE GROWTH-FACTOR IN NORMAL AND PARTIALLY HEPATECTOMIZED RATS
STIMULATION OF LIVER GROWTH BY EXOGENOUS HUMAN HEPATOCYTE GROWTH-FACTOR IN NORMAL AND PARTIALLY HEPATECTOMIZED RATS
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DOI：
10.1016/0270-9139(93)90237-h
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发表时间：
1993-12-01
期刊：
影响因子：
13.5
通讯作者：
KUROKAWA, K
中科院分区：
文献类型：
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作者：
FUJIWARA, K;NAGOSHI, S;KUROKAWA, K
人肝细胞生长因子通过培养的大鼠肝细胞刺激D合成。当从人胚肺成纤维细胞的培养液制备的人肝细胞生长因子静脉注射到正常大鼠和70%肝切除后的大鼠中时，在注射后30 min分离的肝细胞中检测到肝细胞生长因子，但在非实质细胞中未检测到。同样，每隔2小时注射人肝细胞生长因子10小时，48小时后，正常大鼠肝DNA含量显著增加，70%肝切除后肝DNA合成所必需的多胺腐胺含量增加，6小时时腐胺合成催化酶活性几乎达到70%肝切除后的水平。在70%肝切除术后的大鼠中，这些水平通过在手术后立即开始类似的人肝细胞生长因子注射而进一步增强。与人肝细胞生长因子相比，重组人肝细胞生长因子也能在更大程度上增加正常大鼠和70%肝切除后大鼠的肝DNA含量。在正常大鼠给予重组人肝细胞生长因子，5-溴-2 '-脱氧尿苷标记和有丝分裂的肝细胞的数量显着增加，在26小时，但不是在48小时。我们得出结论，外源性人肝细胞生长因子作为一个触发器和肝生长的促进剂，以增加肝腐胺生产大鼠。重组人肝细胞生长因子在此作用中比人肝细胞生长因子更有效。
Human hepatocyte growth factor stimulates D synthesis by cultured rat hepatocytes. When human hepatocyte growth factor prepared from the culture medium of human embryonic lung fibroblasts was intravenously injected into normal rats and rats after 70% hepatectomy, it was detected in hepatocytes but not in nonparenchymal cells isolated 30 min after injection. Similar injections of human hepatocyte growth factor at 2-hr intervals for 10 hr significantly increased hepatic DNA content in normal rats at 48 hr, with increased hepatic content of putrescine, the essential polyamine for hepatic DNA synthesis after 70% hepatectomy, and activities of catalytic enzymes of Putrescine synthesis at 6 hr almost to the levels in rats after 70%-hepatectomy. Those levels in rats after 70% hepatectomy were further enhanced by similar injections of human hepatocyte growth factor starting immediately after surgery. Increased hepatic DNA content in normal rats and rats after 70% hepatectomy was also seen with recombinant human hepatocyte growth factor to a greater extent compared with that seen with human hepatocyte growth factor. In normal rats given recombinant human hepatocyte growth factor, 5-bromo-2'-deoxyuridine-labeled and mitotic hepatocytes were significantly increased in number at 26 hr but not at 48 hr. We conclude that exogenous human hepatocyte growth factor acts as a trigger and a promoter of liver growth to increase hepatic putrescine production in rats. Recombinant human hepatocyte growth factor is more potent- than human hepatocyte growth factor in this action.