Isothermal Self-Assembly of Complex DNA Structures under Diverse and Biocompatible Conditions
Isothermal Self-Assembly of Complex DNA Structures under Diverse and Biocompatible Conditions
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DOI：
10.1021/nl4019512
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发表时间：
2013-09-01
期刊：
影响因子：
10.8
通讯作者：
Yin, Peng
中科院分区：
文献类型：
--
作者：
Myhrvold, Cameron;Dai, Mingjie;Yin, Peng
核酸纳米技术使研究人员能够在体外构建广泛的多维结构。直到最近，大多数基于DNA的结构都是在高镁浓度和非生理环境下通过热退火来组装的。在这里，我们描述了一种DNA自组装系统，它可以在任何规定的温度或均匀的条件下在很大范围内等温地调节形成复杂的靶结构。通过几种不同的方式改变链链相互作用的强度，例如结构域长度、GC含量和结构域之间的连接区，我们能够以可预测的方式在15-69℃之间实现等温组装。我们还观察到在生物相容的条件下，即在生理pH、温度和盐度下，在模拟细胞环境的分子拥挤剂聚乙二醇(PEG)的存在下，某些结构的组装。这代表着朝着体内几何精确的DNA或RNA结构的自组装迈出了重要的一步。
Nucleic acid nanotechnology has enabled researchers to construct a wide range of multidimensional structures in vitro. Until recently, most DNA-based structures were assembled by thermal annealing using high magnesium concentrations and nonphysiological environments. Here, we describe a DNA self-assembly system that can be tuned to form a complex target structure isothermally at any prescribed temperature or homogeneous condition within a wide range. We were able to achieve isothermal assembly between 15 and 69 C in a predictable fashion by altering the strength of strand strand interactions in several different ways, for example, domain length, GC content, and linker regions between domains. We also observed the assembly of certain structures under biocompatible conditions, that is, at physiological pH, temperature, and salinity in the presence of the molecular crowding agent polyethylene glycol (PEG) mimicking the cellular environment. This represents an important step toward the self-assembly of geometrically precise DNA or RNA structures in vivo.