Paracrine regulation of gastric acid secretion by fundic somatostatin.
Paracrine regulation of gastric acid secretion by fundic somatostatin.
复制标题
胃底生长抑素对胃酸分泌的旁分泌调节。
DOI：
10.1152/ajpgi.1987.252.4.g485
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Makhlouf,GM
中科院分区：
文献类型：
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作者：
Schubert,ML;Edwards,NF;Arimura,A;Makhlouf,GM
离体，管腔灌注小鼠胃被用来确定是否生长抑素细胞位于壁细胞附近的酸分泌产生旁分泌的影响。组胺和五肽胃泌素引起的酸分泌伴随着生长抑素分泌的剂量依赖性增加。孵育的胃生长抑素抗血清（最终稀释度1：100），但不与正常血清，增强显着的基础和促分泌素刺激的酸分泌。在次最大刺激下增强最明显（92 +/-18%，P小于0.01，用15 μ M五肽胃泌素和160 +/-6%，P小于0.001，用5 μ M组胺），在最大刺激下最不明显（30 +/-12%，P小于0.05，用200 μ M组胺）。次强场刺激的酸反应（10 V，20 Hz，0.5 ms），伴随着生长抑素分泌的增加，也增加了生长抑素抗血清（115 ± 12%，P <0.01），而对最大场刺激的反应（20 V，20 Hz，0.5 ms），这是伴随着生长抑素分泌的减少，一个典型的胆碱能作用，没有进一步增强。它的结论是，胃底生长抑素调节酸分泌反应的旁分泌（组胺），激素（胃泌素），和神经（乙酰胆碱）的刺激和胆碱能刺激酸分泌反映了乙酰胆碱对壁细胞的直接影响和它的能力，以消除旁分泌抑制生长抑素。
The isolated, luminally perfused mouse stomach was used to determine whether somatostatin cells located in proximity to parietal cells exert a paracrine influence on acid secretion. Acid secretion in response to histamine and pentagastrin was accompanied by a dose-dependent increase in somatostatin secretion. Incubation of the stomach with somatostatin antiserum (final dilution 1:100), but not with normal serum, augmented significantly basal and secretagogue-stimulated acid secretion. The augmentation was most evident with submaximal stimuli (92 +/- 18%, P less than 0.01, with 15 microM pentagastrin and 160 +/- 6%, P less than 0.001, with 5 microM histamine) and least evident with maximal stimuli (30 +/- 12%, P less than 0.05, with 200 microM histamine). The acid response to submaximal field stimulation (10 V, 20 Hz, 0.5 ms), which was accompanied by an increase in somatostatin secretion, was also augmented by somatostatin antiserum (115 +/- 12%, P less than 0.01), whereas the response to maximal field stimulation (20 V, 20 Hz, 0.5 ms), which was accompanied by a decrease in somatostatin secretion, a typically cholinergic effect, was not augmented further. It is concluded that fundic somatostatin modulates the acid secretory response to paracrine (histamine), hormonal (gastrin), and neural (acetylcholine) stimuli and that cholinergic stimulation of acid secretion reflects both the direct effect of acetylcholine on the parietal cell and its ability to eliminate the paracrine restraint exerted by somatostatin.