The analysis of population survey data on DNA sequence variation.
The analysis of population survey data on DNA sequence variation.
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DOI：
10.1093/oxfordjournals.molbev.a040607
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发表时间：
1990-07
影响因子：
10.7
通讯作者：
M. Lynch;T. Crease
中科院分区：
文献类型：
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作者：
M. Lynch;T. Crease
提出了一种技术的分区内和人口之间的组件的情况下，多个人口已被调查的限制性位点的变化的核苷酸多样性。这允许在DNA水平上估计FST的类似物。给出了近似表达式的方差，这些估计产生的核苷酸，个人和人口抽样。应用该技术对几种动物的线粒体DNA和果蝇的几个核基因的现有研究表明，遗传多样性估计的标准误差通常相当大。因此，核苷酸多样性的比较研究需要比目前的标准大得多。通常情况下，只有很小一部分的抽样方差是由个体抽样引起的。即使使用20种左右的限制性内切酶，核苷酸取样也是误差的主要来源，而群体取样往往是相当重要的。一般来说，核苷酸水平上的种群细分程度与单倍型水平上的种群细分程度相当，但由于单倍型之间遗传距离的不平等，确实会出现显著差异。
A technique is presented for the partitioning of nucleotide diversity into within- and between-population components for the case in which multiple populations have been surveyed for restriction-site variation. This allows the estimation of an analogue of FST at the DNA level. Approximate expressions are given for the variance of these estimates resulting from nucleotide, individual, and population sampling. Application of the technique to existing studies on mitochondrial DNA in several animal species and on several nuclear genes in Drosophila indicates that the standard errors of genetic diversity estimates are usually quite large. Thus, comparative studies of nucleotide diversity need to be substantially larger than the current standards. Normally, only a very small fraction of the sampling variance is caused by sampling of individuals. Even when 20 or so restriction enzymes are employed, nucleotide sampling is a major source of error, and population sampling is often quite important. Generally, the degree of population subdivision at the nucleotide level is comparable with that at the haplotype level, but significant differences do arise as a result of inequalities in the genetic distances between haplotypes.