Axon routing across the midline controlled by the Drosophila Derailed receptor
Axon routing across the midline controlled by the Drosophila Derailed receptor
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DOI：
10.1038/990122
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发表时间：
1999-12-02
期刊：
影响因子：
64.8
通讯作者：
Thomas, JB
中科院分区：
文献类型：
--
作者：
Bonkowsky, JL;Yoshikawa, S;Thomas, JB
在关于中线对称的神经系统中，许多神经元将轴突从一侧投射到另一侧。尽管已经确定了控制中线交叉的几个组件(1-4)，但人们对轴突在交叉时如何选择适当的路径知之甚少。例如，在果蝇胚胎中，轴突穿过两个不同束之一的中线，即前连合或后连合（分别为 AC 或 PC）。在这里，我们发现脱轨（Drl）受体酪氨酸激酶由投射到 AC 中的神经元表达，并且在缺乏 Drl 的情况下，此类神经元通常会异常投射到 PC 中。相反，PC 神经元中 Drl 的错误表达迫使它们在 AC 中交叉。 Drl 错误表达神经元的行为和可溶性 Drl 受体探针的体内结合模式表明，Drl 充当 PC 中存在的驱避配体的引导受体。我们的结果表明，Drl 是控制中线交叉的新型成分。
In nervous systems with symmetry about the midline, many neurons project axons from one side to the other. Although several of the components controlling midline crossing have been identified(1-4), little is known about how axons choose the appropriate pathway when crossing. For example, in the Drosophila embryo axons cross the midline in one of two distinct tracts, the anterior or posterior commissure (AC or PC, respectively). Here we show that the Derailed (Drl) receptor tyrosine kinase is expressed by neurons that project in the AC, and that in the absence of Drl such neurons often project abnormally into the PC. Conversely, misexpression of Drl in PC neurons forces them to cross in the AC. The behaviour of Drl-misexpressing neurons and the in vivo binding pattern of a soluble Drl receptor probe indicate that Drl acts as a guidance receptor for a repellent ligand present in the PC. Our results show that Drl is a novel component in the control of midline crossing.