A transcriptome analysis of mitten crab testes (Eriocheir sinensis).
A transcriptome analysis of mitten crab testes (Eriocheir sinensis).
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手指蟹睾丸（Eriocheir sinensis）的转录组分析。
DOI：
10.1590/s1415-47572010005000099
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发表时间：
2011-01
影响因子：
2.1
通讯作者：
Wang Q
中科院分区：
文献类型：
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作者：
Zhang W;Wan H;Jiang H;Zhao Y;Zhang X;Hu S;Wang Q
中华绒螯蟹(Eriocheir Sinensis)性早熟相关基因的鉴定对于更好地了解其生殖发育是至关重要的。为此，我们构建了中华绒毛虫睾丸快速发育期的cDNA文库，并对3388个随机挑选的克隆进行了测序。经过处理后，2990个高质量的表达序列标签(EST)被聚为2415个单基因，包括307个重叠群和2108个单链，然后与NCBI的非冗余(Nr)蛋白质和核苷酸(NT)数据库进行比较，分别使用Blastx和Blastn进行注释。经过进一步分析，获得了922个带有具体注释的非基因，其中30个非基因可能与雄性蟹的生殖过程有关-6个与精子发生有关的转录本(特别是Cyclin K和RecA同源物DMC1)，2个与核蛋白转换有关的转录本，2个热休克蛋白基因，11个转录因子基因(一系列锌指蛋白)，9个细胞骨架蛋白相关基因。我们的结果除了提供与甲壳类繁殖有关的有价值的信息外，还可以作为未来生殖和发育生物学研究的基础。
The identification of expressed genes involved in sexual precocity of the mitten crab (Eriocheir sinensis) is critical for a better understanding of its reproductive development. To this end, we constructed a cDNA library from the rapid developmental stage of testis of E. sinensis and sequenced 3,388 randomly picked clones. After processing, 2,990 high-quality expressed sequence tags (ESTs) were clustered into 2,415 unigenes including 307 contigs and 2,108 singlets, which were then compared to the NCBI non-redundant (nr) protein and nucleotide (nt) database for annotation with Blastx and Blastn, respectively. After further analysis, 922 unigenes were obtained with concrete annotations and 30 unigenes were found to have functions possibly related to the process of reproduction in male crabs – six transcripts relevant to spermatogenesis (especially Cyclin K and RecA homolog DMC1), two transcripts involved in nuclear protein transformation, two heat-shock protein genes, eleven transcription factor genes (a series of zinc-finger proteins), and nine cytoskeleton protein-related genes. Our results, besides providing valuable information related to crustacean reproduction, can also serve as a base for future studies of reproductive and developmental biology.