Rules for RNA recognition of GNRA tetraloops deduced by in vitro selection: Comparison with in vivo evolution
Rules for RNA recognition of GNRA tetraloops deduced by in vitro selection: Comparison with in vivo evolution
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DOI：
10.1093/emboj/16.11.3289
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发表时间：
1997-06-02
期刊：
影响因子：
11.4
通讯作者：
Michel, F
中科院分区：
文献类型：
--
作者：
Costa, M;Michel, F
具有GNRA共有序列的末端环是大的自组装RNA分子的突出特征。为了研究涉及GNRA环的三级相互作用，我们设计了一个来自I组核酶的体外选择系统，两个选择，旨在分离能够识别两个最广泛的环的RNA序列（GUGA和GAAA），产生了先前鉴定的那些环的受体的变体，以及一些尚未识别的对GNRA家族成员具有新特异性的高亲和力结合剂，通过利用现有的晶体结构，我们试图根据RNA-RNA接触来合理化这些结果，并揭示一些控制GNRA环介导的三级相互作用的结构原理;强调了环核苷酸2在确保受体特异性识别中的作用，更一般地，体外和自然选择的产物的比较显示，提供了深入了解自组装RNA分子在体内进化的机制。
Terminal loops with a GNRA consensus sequence are a prominent feature of large self-assembling RNA molecules. In order to investigate tertiary interactions involving GNRA loops, we have devised an in vitro selection system derived from a group I ribozyme, Two selections, destined to isolate RNA sequences that would recognize two of the most widespread loops (GUGA and GAAA), yielded variants of previously identified receptors for those loops, and also some yet unrecognized, high-affinity binders with novel specificities towards members of the GNRA family, By taking advantage of available crystal structures, we have attempted to rationalize these results in terms of RNA-RNA contacts and to expose some of the structural principles that govern GNRA loop-mediated tertiary interactions; the role of loop nucleotide 2 in ensuring specific recognition by receptors is emphasized, More generally, comparison of the products of in vitro and natural selection is shown to provide insights into the mechanisms underlying the in vivo evolution of self-assembling RNA molecules.