The binding of staphylococcal protein A by the sera of different animal species.
The binding of staphylococcal protein A by the sera of different animal species.
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DOI：
10.4049/jimmunol.128.5.2300
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发表时间：
1982-05
影响因子：
4.4
通讯作者：
D. Richman;P. Cleveland;M. Oxman;K. Johnson
中科院分区：
文献类型：
--
作者：
D. Richman;P. Cleveland;M. Oxman;K. Johnson
纯化的免疫球蛋白或血清结合 (125I) 标记的葡萄球菌蛋白 A (SPA) 的能力通过免疫过滤测定法进行测量，该测定法有助于检查大量血清，并且每种血清仅需微量。对 80 个物种的血清进行了检查，包括人类、实验动物、家畜和各种非洲哺乳动物。血清免疫球蛋白的 SPA 结合能力存在广泛的种间差异。除一个明显的例外外，同一物种内的个体之间仅观察到微小的差异。在 9 只山羊的血清中观察到 SPA 结合能力存在超过 10,000 倍的变化。血清 SPA 结合能力的种间差异与分类差异密切相关，血清 SPA 结合能力与分类差异密切相关，非洲哺乳动物的血清 SPA 结合能力与其更常见的近亲密切相关。间接 (125I)SPA 免疫测定法检测抗体的灵敏度主要取决于所检测血清的 SPA 结合能力。通过(125I)SPA免疫滤过法测定的几种不同物种血清中的甲型/德克萨斯/1/77(H3N2)流感病毒抗体滴度与血清的SPA结合能力成正比，与血凝抑制滴度不成正比。对 Mastomys natalensis 血清中的拉沙病毒抗体进行 (125I)SPA 免疫过滤测定（其结合 SPA 的能力仅为人血清的千分之一）至少与标准荧光抗体测定一样灵敏。
The capacity of purified immunoglobulin or serum to bind (125I)-labeled staphylococcal protein A (SPA) was measured by means of an immunofiltration assay that facilitated the examination of large numbers of sera and required only a minute quantity of each. Sera from 80 species, including humans, laboratory animals, domestic animals, and a variety of African mammals were examined. A wide interspecies variation in the SPA-binding capacity of serum immunoglobulins was confirmed. Only small variations were observed among individuals within the same species with one notable exception. A greater than 10,000-fold variation in SPA-binding capacity was observed among sera from nine goats. Interspecies differences in serum SPA-binding capacity correlated well with taxonomic differences, and the serum SPA-binding capacity correlated well with taxonomic differences, and the serum SPA-binding capacities of African mammals corresponded closely to those of their more common relatives. The sensitivity of antibody detection by indirect (125I)SPA immunoassay was shown to be determined mostly by the SPA-binding capacity of the serum examined. The titer of antibody to influenza A/Texas/1/77(H3N2) virus in sera from several different species, when measured by (125I)SPA immunofiltration assay, was directly proportional to the SPA-binding capacity of the serum and was not proportional to the hemagglutination inhibition titer. An (125I)SPA immunofiltration assay for antibody to Lassa virus in the serum of Mastomys natalensis (which binds SPA only one-thousandth as well as human serum) was at least as sensitive as the standard fluorescent antibody assay.