Author Correction: Amylin and pramlintide modulate γ-secretase level and APP processing in lipid rafts.
Author Correction: Amylin and pramlintide modulate γ-secretase level and APP processing in lipid rafts.
复制标题
作者更正：胰淀素和普兰林肽调节脂筏中的γ分泌酶水平和APP加工。
DOI：
10.1038/s41598-020-68281-y
复制
发表时间：
2020
影响因子：
4.6
通讯作者：
Kaddoumi,Amal
中科院分区：
文献类型：
--
作者：
Mousa,YoussefM;Abdallah,IhabM;Hwang,Misako;Martin,DouglasR;Kaddoumi,Amal
图1.胰淀素和普兰林肽对全脑匀浆中APP加工的影响。(a)全长APP（fAPP）和BACE 1的代表性蛋白质印迹和光密度分析表明，胰淀素和普兰林肽不会改变小鼠脑匀浆中的全长APP（fAPP）和BACE 1。将fAPP和BACE 1水平标准化为GAPDH水平。(b)小鼠脑中sAPP-β和sAPP-α的代表性蛋白质印迹和光密度分析表明，与对照组相比，普兰林肽显著增加sAPP-β，而用胰淀素和普兰林肽处理后观察到sAPP-α水平降低。将sAPP-β和sAPP-α的水平标准化为β-微管蛋白的水平。由于分子量相似，sAPP-β和sAPP-α在不同的凝胶上运行。(c)小鼠脑中γ-分泌酶亚基的代表性蛋白质印迹和光密度分析表明，与对照和胰淀素相比，普兰林肽导致PEN 2亚基显著增加;然而，两种肽均不影响其他γ-分泌酶亚基PSEN 1、PSEN 2和nicastrin。将γ-分泌酶亚基的水平归一化为每个相应泳道中的GAPDH水平。由于分子量相似，PSEN 1和PSEN 2在不同的凝胶上运行。(d)小鼠脑中β-CTF的代表性蛋白质印迹和光密度分析表明，与对照相比，胰淀素和普兰林肽导致β-CTF显著增加。数据表示为平均值土SEM，并且密度测定分析来自每组n= 6只小鼠。蛋白质印迹结果是来自每组的两只不同小鼠的代表性结果。数据表示为平均值±SEM，所有结果的统计学显著性通过学生t检验评估，与对照组相比，* p< 0.05，** p <0.01，* p< 0.001;与普兰林肽相比，# p< 0.05，ns=不显著。
Figure 1. Effect of amylin and pramlintide on APP processing in total brain homogenate.(a) Representative Western blot and densitometry analysis of full-length APP (fAPP) and BACE1 demonstrated amylin and pramlintide did not alter full-length APP (fAPP) and BACE1 in mice brain homogenates. The fAPP and BACE1 levels were normalized to GAPDH level.(b) Representative Western blot and densitometry analysis of sAPP-β and sAPP-α in mice brains demonstrated pramlintide significantly increased sAPP-β compared to control, whereas reduction in the level of sAPP-α was observed after treatment with amylin and pramlintide. The levels of sAPP-β and sAPP-α were normalized to the level of β-tubulin. sAPP-β and sAPP-α were ran on different gels due to molecular weight similarity.(c) Representative Western blot and densitometry analysis of γ-secretase subunits in mice brains demonstrated pramlintide caused a significant increase in PEN2 subunit when compared to control and amylin; however, neither peptide influenced the other γ-secretase subunits PSEN1, PSEN2 and nicastrin. The level of γ-secretase subunits was normalized to level of GAPDH in each corresponding lane. PSEN1 and PSEN2 were ran on different gels due to molecular weight similarity.(d) Representative Western blot and densitometry analysis of β-CTF in mice brains demonstrated amylin and pramlintide caused a significant increase in β-CTF when compared to control. Data is presented as mean±SEM, and the densitometry analysis is from n= 6 mice in each group. The western blot results are representative results from two different mice from each group. Data is presented as mean±SEM and the statistical significance for all result was assessed by student t-test, with* p< 0.05,** p< 0.01,*** p< 0.001 compared to control group;# p< 0.05 compared to pramlintide, and ns= not significant.