Ethanol extract of Crocus sativus L. Antagonizes the inhibitory action of ethanol on hippocampal long‐term potentiation in vivo
Ethanol extract of Crocus sativus L. Antagonizes the inhibitory action of ethanol on hippocampal long‐term potentiation in vivo
复制标题
番红花乙醇提取物拮抗乙醇对体内海马长时程增强的抑制作用
DOI：
10.1002/ptr.2650090204
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发表时间：
1995
影响因子：
7.2
通讯作者：
Y. Shoyama
中科院分区：
文献类型：
--
作者：
M. Sugiura;H. Saito;K. Abe;Y. Shoyama
西红花乙醇提取物对小鼠抗氧化作用的影响。(CSE)对海马齿状回诱发电位长时程增强（LTP）的影响。CSE（250 mg/kg，p.o.）单独使用不影响通过应用阈下或阈上强直刺激（60 Hz下20或30个脉冲）产生LTP。经口给予乙醇（10- 30%，10 mL/kg）可阻断由60 Hz 30次脉冲强直刺激诱导的LTP，但预先给予CSE（125和250 mg/kg，p.o.）可显著减弱乙醇的LTP阻断作用。静脉注射乙醇（30%，2 mL/kg）对LTP诱导的阻断作用也可通过给予125和250 mg/kg（p.o.）剂量的CSE拮抗。CSE经口给药也拮抗了直接注射到脑中的乙醇的LTP阻断作用，尽管它需要更高的剂量（500 mg/kg，p.o.）。这些结果表明，口服CSE对乙醇诱导的海马突触可塑性损伤具有拮抗作用，这可能是通过对中枢神经系统的直接作用和外周作用介导的。
The effect of an ethanol extract of Crocus sativus L. (CSE) on the long‐term potentiation (LTP) of the evoked potential in the dentate gyrus of the hippocampus was investigated using anaesthetized rats. CSE (250 mg/kg, p.o.) alone did not affect the generation of LTP by application of subthreshold or suprathreshold tetanic stimulation (20 or 30 pulses at 60 Hz). Oral administration of ethanol (10–30%, 10 mL/kg) blocked the LTP induced by tetanic stimulation of 30 pulses at 60 Hz, but the LTP‐blocking effect of ethanol was significantly attenuated by pre‐administration of CSE (125 and 250 mg/kg, p.o.). The blockade of LTP induction by intravenously injected ethanol (30%, 2 mL/kg) was also antagonized by administration of CSE at doses of 125 and 250 mg/kg (p.o.). Oral administration of CSE antagonized also the LTP‐blocking effect of ethanol directly injected into the brain, although it required a higher dose (500 mg/kg, p.o.). These results suggest that oral administration of CSE exerts an antagonistic effect on ethanol‐induced impairment of hippocampal synaptic plasticity, which is possibly mediated by both direct action on the central nervous system and peripheral action.