Selective regulation by pertussis toxin of insulin-induced activation of particulate cAMP phosphodiesterase activity in 3T3-L1 adipocytes.
Selective regulation by pertussis toxin of insulin-induced activation of particulate cAMP phosphodiesterase activity in 3T3-L1 adipocytes.
复制标题
百日咳毒素选择性调节胰岛素诱导的 3T3-L1 脂肪细胞中颗粒 cAMP 磷酸二酯酶活性的激活。
DOI：
10.1016/0006-291x(83)90565-x
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发表时间：
1983
影响因子：
3.1
通讯作者：
Vaughan,M
中科院分区：
文献类型：
--
作者：
Elks,ML;Watkins,PA;Manganiello,VC;Moss,J;Hewlett,E;Vaughan,M
Incubation of 3T3-L1 adipocytes with insulin or isoproterenol for 10 min increased particulate “low Km” cAMP phosphodiesterase activity by 42% and 50%, respectively. Pertussis toxin catalyzed the [32P]-ADP ribosylation of a 41,000 dalton protein in adipocyte particulate fractions; prior incubation of adipocytes with toxin markedly reduced incorporation of radiolabel. Exposure of adipocytes to pertussis toxin (0.3 μg, 18 hr) increased glycerol production and inhibited activation of cAMP phosphodiesterase by insulin, but not by isoproterenol. These results suggest that pertussis toxin can interfere with receptor-mediated processes that stimulate cAMP hydrolysis as well as those that inhibit cAMP formation.
