A dendrimer-based highly sensitive and selective fluorescence quenching sensor for Fe3+ both in solution and as film
A dendrimer-based highly sensitive and selective fluorescence quenching sensor for Fe3+ both in solution and as film
复制标题
基于树枝状聚合物的高灵敏度、选择性荧光猝灭传感器，适用于溶液和薄膜中的 Fe3
DOI：
10.1016/j.bios.2016.05.046
复制
发表时间：
2016
影响因子：
12.6
通讯作者：
Muellen Klaus
中科院分区：
文献类型：
--
作者：
Li Peng;Zhang Ming;Sun Xueke;Guan Shuwen;Zhang Guang;Baumgarten Martin;Muellen Klaus
以芘为内核，三苯胺（TPA）为外围的新型荧光树枝状聚合物PYTPAG 2，研究了其作为铁离子（Fe 3+）荧光猝灭传感器的溶液和薄膜两种形式.这种基于树枝状聚合物的传感器具有优先检测Fe 3+的非常强的荧光猝灭没有发现其他金属离子。该传感器对溶液和薄膜中Fe ~（3+）的荧光检测限分别为6.5×10 ~（-7）M和5.0×10 ~（-7）M。这一过程的可能机制是由于PYTPAG 2的TPA单元与Fe ~（3+）离子发生络合作用，破坏了TPA基团向芘核（PYTPAG 2分子内）的荧光共振能量转移（FRET），导致荧光猝灭。此外，这种惊人的性能不会受到pH值的干扰，与其他金属离子，抗衡阴离子，或周围环境。此外，活体蚯蚓体内Fe ~（3+）的生物荧光成像研究也证明了其具有重要的实际应用价值。
A novel fluorescent dendrimer PYTPAG2, with pyrene as the interior core and triphenylamine (TPA) as the exterior periphery, is studied as a fluorescence-quenching sensor for iron (ш) ions (Fe3+), both in solution and as a film. This dendrimer-based sensor possesses preferential detection ofFe3+by a very strong fluorescence quenching not found for other metal ions. The fluorescent detection limits of this PYTPAG2 sensor forFe3+in solution and thin-film are 6.5×10−7M and 5.0×10−7M, respectively. The possible mechanism of this process is explained by the complexation between the peripheral TPA units of PYTPAG2 andFe3+ions, which may disrupt the fluorescence resonance energy transfer (FRET) from the TPA groups to the pyrene core (intramolecular of PYTPAG2) and results in the fluorescence quenching. Moreover, this striking performance could not be disturbed by pH, the interference with other metal ions, counter anions, or surrounding environment. In addition, biological fluorescence imaging studies ofFe3+in living roundworms demonstrate its valuable practical application.