Distribution and characterization of plasmalemma vesicle protein-1 in rat endocrine glands
Distribution and characterization of plasmalemma vesicle protein-1 in rat endocrine glands
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DOI：
10.1677/joe.0.1750649
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发表时间：
2002-12-01
影响因子：
4
通讯作者：
Ben-Jonathan, N
中科院分区：
文献类型：
--
作者：
Hnasko, R;McFarland, M;Ben-Jonathan, N
质膜囊泡蛋白-1 （PV-1）是一种与内皮细胞小泡和小孔相关的完整膜蛋白。由于内分泌腺富含开孔内皮，我们检测了PV-1 mRNA和蛋白在内分泌腺中的分布，并确定了其细胞定位。RT-PCR在所有检测的内分泌腺中检测到单一转录本。合成肽用于产生抗体用于免疫印迹和免疫组化（IHC）。肺、垂体、肾上腺、睾丸和pv -1转染的Cos-1细胞的膜组分Western blotting显示一个主要的65 kDa蛋白。这种蛋白以高亲和力与肝素结合。通过免疫组化，PV-1定位于肾上腺网状带内皮细胞和髓质的染色质细胞。在胰腺中，PV-1的表达仅限于朗格汉斯胰岛中的少数细胞，这些细胞与生长抑素阳性的δ细胞部分重叠。在新生儿和成人垂体中，在垂体神经叶中检测到很强的PV-1免疫反应性，其模式与胶质纤维酸性蛋白（GFAP）相似。PV-1和GFAP在成人中间叶中表达，而在新生儿中间叶中未见表达。新生儿前叶的内皮细胞均呈PV-1阳性，但成人的PV-1仅限于叶边缘附近的一些内皮细胞和内分泌细胞。在成年睾丸中，PV-1在精管内的生殖细胞中表达强烈，随精子发生的阶段而变化。相比之下，新生儿睾丸中的PV-1定位于间质细胞而不是精管。在卵巢中，PV-1在基质内皮细胞和发育卵泡的鞘层中表达。半数以上黄体细胞呈PV-1阳性。我们的数据表明PV-1并不局限于内皮细胞，而是局限于许多类型的内分泌和非内分泌细胞。此外，PV-1在垂体和睾丸中的表达受发育调控。
Plasmalemma vesicle protem-1 (PV-1) is an integral membrane protein associated with endothelial cell caveolae and fenestrae. Since endocrine glands are enriched with fenestrated endothelium, we examined the distribution of PV-1 mRNA and protein in endocrine glands and determined its cellular localization. A single transcript was detected by RT-PCR in all endocrine glands examined. A synthetic peptide was used to generate antibodies for Western blotting and immunohistochemistry (IHC). Western blotting of membrane fractions from lung, pituitary, adrenal, testis and PV-1-transfected Cos-1 cells revealed a major 65 kDa protein. This protein binds to heparin with high affinity. Using IHC, PV-1 was localized to both endothelial cells of the adrenal zona reticularis and chromaffin cells of the medulla. In the pancreas, PV-1 expression was restricted to a few cells in the islets of Langerhans that partially overlap with somatostatin-positive delta-cells. In both neonatal and adult pituitaries, strong PV-1 immunoreactivity was detected in neural lobe pituicytes in a pattern similar to that of glial fibrillary acidic protein (GFAP). PV-1 and GFAP expression was seen in the adult, but not neonatal, intermediate lobe. Endothelial cells throughout the neonatal anterior lobe were PV-1 positive, but PV-1 in the adult was restricted to some endothelial and endocrine cells localized near the margins of lobe. In the adult testis, strong PV-1 expression was seen in germ cells within the seminiferous tubules that varied with the stage of spermatogenesis. In contrast, PV-1 in the neonatal testis was localized to the interstitial cells but not seminiferous tubules. In the ovary, PV-1 was expressed in stromal endothelial cells as well as the thecal layer of developing follicles. Over half the corpus luteal cells were positive for PV-1. Our data have shown that PV-1 is not restricted to endothelial cells but is localized in many types of endocrine and non-endocrine cells. Furthermore, PV-1 expression in the pituitary and testis is developmentally regulated.