Metastatic small cell malignant melanoma: A case requiring immunoelectronmicroscopy for the demonstration of lattice-deficient melanosomes
Metastatic small cell malignant melanoma: A case requiring immunoelectronmicroscopy for the demonstration of lattice-deficient melanosomes
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DOI：
10.1080/01913120590910612
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发表时间：
2005-01-01
影响因子：
1
通讯作者：
Banerjee, SS
中科院分区：
文献类型：
--
作者：
Eyden, B;Moss, J;Banerjee, SS
转移性恶性黑色素瘤的情况下，表现出小细胞形态描述。患者既往患有原发性结节性小细胞黑素瘤。常规组织学、光镜免疫组化、常规电镜和超微结构免疫标记检查转移瘤。它由小细胞组成，但大小不一，存在于不同但合并的区域。肿瘤细胞S-100蛋白阴性，细胞角蛋白灶性阳性：这些结果结合小细胞形态提示小细胞癌的可能性。然而，其他黑素细胞市场表现积极。神经内分泌市场是负面的。通过电子显微镜，肿瘤细胞缺乏明确的黑素体，但含有非描述性颗粒的核旁聚集体。在超微结构免疫标记后，发现这些被金标记的HMB-45抗体修饰，从而证实它们是晶格缺陷型黑素体。这种肿瘤是恶性黑色素瘤的一个罕见的例子，免疫电镜分析有助于澄清其他无法描述的颗粒的性质，作为真正的，但晶格缺陷的黑素体。这也是第一个通过电子显微镜研究的小细胞黑素瘤病例。
A case of metastatic malignant melanoma exhibiting small cell morphology is described. The patient had had a previous primary nodular small cell melanoma. The metastatic tumor was examined by conventional histology, light microscope immunohistochemistry, conventional electron microscopy, and Ultrastructural immunolabeling. It consisted of small cells, which, however, varied in size and were present in distinct but merging areas. Tumor cells were negative for S-100 protein and very focally positive for cytokeratin: these findings in combination with small cell morphology Suggested the possibility of small cell carcinoma. However, other melanocytic markets were positive. Neuroendocrine markets were negative. By electron microscopy, tumor cells lacked unambiguous melanosomes but contained paranuclear aggregates of nondescript granules. Following ultrastructural immunolabeling, these were found to be decorated with gold-labeled HMB-45 antibodies, thereby confirming them as lattice-deficient melanosomes. This tumor is an uncommon example of malignant melanoma where immunoultrastructural analysis helped clarify the nature of otherwise nondescript granules as true but lattice-deficient melanosomes. This is also the first case of small cell melanoma to be studied by electron microscopy.