A near-infrared fluorescent probe for monitoring fluvastatin-stimulated endogenous H2S production
A near-infrared fluorescent probe for monitoring fluvastatin-stimulated endogenous H2S production
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DOI：
10.1016/j.cclet.2016.07.008
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发表时间：
2017-02
影响因子：
9.1
通讯作者：
Lili Zhang;Huikun Zhu;Chunchang Zhao;Xianfeng Gu
中科院分区：
文献类型：
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作者：
Lili Zhang;Huikun Zhu;Chunchang Zhao;Xianfeng Gu
由于强烈的自发荧光背景，大多数报道的荧光探针在生命系统的实际应用中都存在局限性，构建具有近红外（NIR）荧光发射的探针是解决这一挑战的一种可行方法。我们设计了一种近红外荧光探针，用于监测活细胞中内源性 H2S 的产生。设计的探针对H2S表现出显着的近红外荧光开启响应，具有高选择性，能够灵敏地检测H2S。重要的是，该探针可用于监测 raw264.7 巨噬细胞中内源性 H2S 的产生。本研究表明氟伐他汀可以促进胱硫醚γ-裂解酶（CSE）产生H2S的活性。
Most reported fluorescent probes have limitations in practical applications in living systems due to the strong autofluorescence background, construction of probes with near-infrared (NIR) fluorescence emission is an accessible approach for addressing this challenge. We here designed a NIR fluorescent probe for monitoring the endogenous production of H2S in living cells. The designed probe showed significant NIR fluorescence turn-on response to H2S with high selectivity, enabling the sensitive detection H2S. Importantly, the probe could be applied in monitoring the endogenous production of H2S in raw264.7 macrophages. This study showed that fluvastatin can promote the activity of cystathionineγ-lyase (CSE) for generation H2S.