Near-infrared spectroscopic characterization of human advanced atherosclerotic plaques
Near-infrared spectroscopic characterization of human advanced atherosclerotic plaques
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DOI：
10.1016/s0735-1097(02)01767-9
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发表时间：
2002-04-17
影响因子：
24
通讯作者：
Casscells, W
中科院分区：
文献类型：
--
作者：
Wang, J;Geng, YJ;Casscells, W
目的 通过近红外 (NIR) 光谱在体外检查活体人类颈动脉粥样硬化斑块，以确定斑块脆弱性的光谱特征。 背景 斑块破坏是心脏病发作和中风的主要原因，通常无法预测，但被认为主要取决于斑块成分。近红外光谱已被用于非侵入性地检测组织中的成分。方法使用装有光纤探头的近红外光谱仪，对活体人颈动脉粥样硬化斑块（来自 25 名患者）进行离体检查以了解斑块的脆弱性。根据大体病理特征将斑块切成较小的切片，并在20℃下进行NIR测量，通常在10分钟内进行。 结果根据美国心脏协会推荐的分类方案，将病变分为三组：第一组为易损tvpe V/VI病变；第二组为易损tvpe V/VI病变；第二组，稳定的tvpe I/II病灶；第三组（中间），主要是III/IV型病变。对样本的 NIR 光谱进行聚类分析，确定了三个 NIR 光谱区域中每个区域的三个主要 p 成分组：2,200 至 2,330 nm、1,620 至 1,820 nm 和 1,130 至 1,260 nm。在两个 NIR 区域（2,200 至 2,330 nm）计算每组中的脂质/蛋白质比率，显示 I/II 型、III/IV 型和晚期 V/VI 型病变的比率分别为 1.49 +/- 1.20、2.12 +/- 1.00 和 3.37 +/- 0.88（p < 0.03）。在 1,620 至 1,820 nm 处，这些组织学组的相应比率为 0.57 +/- 0.21、1.54 +/- 0.46 和 2.40 +/- 0.44 (p < 0.00003)。结论 该螺柱中组织学易损斑块和稳定斑块的良好离体区分表明近红外光谱有可能识别易损斑块体内动脉粥样硬化斑块。 (C) 2002 年由美国心脏病学会基金会资助。
OBJECTIVES Living human carotid atherosclerotic plaques were examined in vitro by near-infrared (NIR) spectroscopy to determine the spectral features of plaque vulnerability.BACKGROUND Plaque disruption, a major cause of heart attacks and strokes, cannot generally be predicted, but is thought to depend mainly on plaque composition. Near-infrared spectroscopy has been used to detect components in tissues noninvasively.METHODS Using an NIR spectrometer fitted with a fiberoptic probe, living human carotid atherosclerotic plaques (from 25 patients) were examined ex vivo for plaque vulnerability. The plaques were cut into smaller sections according to their gross pathologic features, and NIR measurements were done at 20degreesC, usually within 10 min,RESULTS According to the American Heart Association's recommended classification scheme, the lesions were classified into three groups: the first group comprised of vulnerable tvpe V/VI lesions; the second group, stable tvpe I/II lesions; and the third (intermediate) group, mainly type III/IV lesions. Cluster analysis of the specimens' NIR spectra identified three major p composition groups in each of the three NIR spectral regions: 2,200 to 2,330 nm, 1,620 to 1,820 nm and 1,130 to 1,260 nm. Calculation of the lipid/protein ratios in each group at two NIR regions (2,200 to 2,330 nm) revealed ratios of 1.49 +/- 1.20, 2.12 +/- 1.00 and 3.37 +/- 0.88 for type I/II, type III/IV and advanced type V/VI lesions, respectively (p < 0.03). At 1,620 to 1,820 nm, the respective ratios for these histologic groups were 0.57 +/- 0.21, 1.54 +/- 0.46 and 2.40 +/- 0.44 (p < 0.00003).CONCLUSIONS The good ex vivo discrimination of histologically vulnerable and stable plaques in this studs suggests that NIR spectroscopy has the potential to identify vulnerable atherosclerotic plaques in vivo. (C) 2002 by the American College of Cardiology Foundation.