Cutting edge:: Identification of E-cadherin as a ligand for the murine killer cell lectin-like receptor G1
Cutting edge:: Identification of E-cadherin as a ligand for the murine killer cell lectin-like receptor G1
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DOI：
10.4049/jimmunol.176.3.1311
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发表时间：
2006-02-01
影响因子：
4.4
通讯作者：
Pircher, H
中科院分区：
文献类型：
--
作者：
Gründemann, C;Bauer, M;Pircher, H
杀伤细胞凝集素样受体G1（KERG 1）由NK细胞和T细胞表达。在人类和小鼠中，KLRG 1鉴定了增殖能力受损但能够发挥作用或功能的Ag经历的T细胞。在这项研究中，我们确定了E-钙粘蛋白作为配体的鼠KLRG 1通过使用荧光标记，可溶性四聚体复合物的细胞外结构域的鼠KLRG 1分子作为染色试剂的表达克隆。B16.BL6靶细胞中E-cadherin的异位表达不影响淋巴因子激活的NK细胞和CD 8 T细胞介导的细胞溶解，但抑制Ag诱导的增殖和诱导的CD 8 T细胞的细胞溶解活性。E-钙粘蛋白由正常上皮细胞、朗格汉斯细胞和角质形成细胞表达，并且通常在转移性癌细胞中下调。因此，在健康组织中通过E-钙粘蛋白连接KLRG 1可能对致敏的T细胞产生抑制作用。免疫学杂志，2006年。
The killer cell lectin-like receptor G1 (KERG1) is expressed by NK cells and by T cells. In both humans and mice, KLRG1 identifies Ag-experienced T cells that are impaired in their proliferative capacity but are capable of performing effect or functions. In this study, we identified E-cadherin as a ligand for murine KLRG1 by using fluorescently labeled, soluble tetrameric complexes of the extracellular domain of the murine KLRG1 molecule as staining reagents in expression cloning. Ectopic expression of E-cadherin in B16.BL6 target cells did not affect cell-mediated lysis by lymphokine-activated NK cells and by CD8 T cells but inhibited Ag-induced proliferation and induction of cytolytic activity of CD8 T cells. E-cadherin is expressed by normal epithelial cells, Langerhans cells, and keratinocytes and is usually down-regulated on metastatic cancer cells. KLRG1 ligation by E-cadherin in healthy tissue may thus exert an inhibitory effect on primed T cells. The Journal of Immunology, 2006.